Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Apr;65(4):1017–1024. doi: 10.1073/pnas.65.4.1017

DNA Replication in Mammalian Cells, I. The Size of Newly Synthesized Helices

Fiorella Nuzzo 1,2, Agnese Brega 1,2, Arturo Falaschi 1,2
PMCID: PMC283017  PMID: 4985879

Abstract

The newly synthesized DNA of heteroploid human cells cultured in vitro has a single-stranded molecular weight of approximately 1 × 105 following 30- to 60-second pulses of labeling with tritiated thymidine. With increasing pulse times the amount of radioactive DNA having a low single-stranded molecular weight remains approximately constant, whereas increasing amounts of label are found associated with the bulk DNA. After removing the label from the medium, the radioactivity associated with the small molecular weight DNA is chased into high molecular weight material. These findings are analogous to what is observed in microorganisms and suggest a basically common organization of the growing point of DNA in bacterial and in mammalian cells.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  2. Hosoda J., Mathews E. DNA replication in vivo by a temperature-sensitive polynucleotide ligase mutant of T4. Proc Natl Acad Sci U S A. 1968 Nov;61(3):997–1004. doi: 10.1073/pnas.61.3.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Huberman J. A., Riggs A. D. On the mechanism of DNA replication in mammalian chromosomes. J Mol Biol. 1968 Mar 14;32(2):327–341. doi: 10.1016/0022-2836(68)90013-2. [DOI] [PubMed] [Google Scholar]
  4. Kidson C. Analysis of DNA fine secondary structure in relation to replication and transcription. Cold Spring Harb Symp Quant Biol. 1968;33:179–186. doi: 10.1101/sqb.1968.033.01.021. [DOI] [PubMed] [Google Scholar]
  5. Lehmann A. R., Ormerod M. G. Artefact in the measurement of the molecular weight of pulse labelled DNA. Nature. 1969 Mar 15;221(5185):1053–1056. doi: 10.1038/2211053b0. [DOI] [PubMed] [Google Scholar]
  6. Newman J., Hanawalt P. Role of polynucleotide ligase in T4 DNA replication. J Mol Biol. 1968 Aug 14;35(3):639–642. doi: 10.1016/s0022-2836(68)80020-8. [DOI] [PubMed] [Google Scholar]
  7. Oishi M. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA. Proc Natl Acad Sci U S A. 1968 May;60(1):329–336. doi: 10.1073/pnas.60.1.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Okazaki R., Okazaki T., Sakabe K., Sugimoto K., Sugino A. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains. Proc Natl Acad Sci U S A. 1968 Feb;59(2):598–605. doi: 10.1073/pnas.59.2.598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Painter R. B., Schaefer A. State of newly synthesized HeLa DNA. Nature. 1969 Mar 29;221(5187):1215–1217. doi: 10.1038/2211215a0. [DOI] [PubMed] [Google Scholar]
  10. Richardson C. C., Masamune Y., Live T. R., Jacquemin-Sablon A., Weiss B., Fareed G. C. Studies on the joining of DNA by polynucleotide ligase of phage T4. Cold Spring Harb Symp Quant Biol. 1968;33:151–164. doi: 10.1101/sqb.1968.033.01.019. [DOI] [PubMed] [Google Scholar]
  11. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  12. Sugimoto K., Okazaki T., Okazaki R. Mechanism of DNA chain growth, II. Accumulation of newly synthesized short chains in E. coli infected with ligase-defective T4 phages. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1356–1362. doi: 10.1073/pnas.60.4.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TERNI M., LO MONACO G. B. Coltura continua di cellule derivate da embrione umano. Sperimentale. 1958 May-Jun;108(3):177–185. [PubMed] [Google Scholar]
  14. Taylor J. H. Rates of chain growth and units of replication in DNA of mammalian chromosomes. J Mol Biol. 1968 Feb 14;31(3):579–594. doi: 10.1016/0022-2836(68)90429-4. [DOI] [PubMed] [Google Scholar]
  15. Tsukada K., Moriyama T., Lynch W. E., Lieberman I. Polydeoxynucleotide intermediates in DNA replication in regenerating liver. Nature. 1968 Oct 12;220(5163):162–164. doi: 10.1038/220162a0. [DOI] [PubMed] [Google Scholar]
  16. Yudelevich A., Ginsberg B., Hurwitz J. Discontinuous synthesis of DNA during replication. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1129–1136. doi: 10.1073/pnas.61.3.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES