Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jun;66(2):495–499. doi: 10.1073/pnas.66.2.495

Synthesis of Acid Mucopolysaccharides by Glial Tumor Cells in Tissue Culture*

Albert Dorfman 1,2,3,, Pei-Lee Ho 1,2,3
PMCID: PMC283072  PMID: 4247492

Abstract

A clonal strain of rat glial tumor cells was shown to produce the acid mucopolysaccharides, hyaluronic acid, chondroitin 4-SO4, and heparan sulfate in tissue culture. The mucopolysaccharides were isolated both from the growth medium and the cells.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABOOD L. G., ABUL-HAJ S. K. Histochemistry and characterization of hyaluronic acid in axons of peripheral nerve. J Neurochem. 1956 Dec;1(2):119–125. doi: 10.1111/j.1471-4159.1956.tb12062.x. [DOI] [PubMed] [Google Scholar]
  2. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  3. Benda P., Lightbody J., Sato G., Levine L., Sweet W. Differentiated rat glial cell strain in tissue culture. Science. 1968 Jul 26;161(3839):370–371. doi: 10.1126/science.161.3839.370. [DOI] [PubMed] [Google Scholar]
  4. CLAUSEN J., HANSEN A. Acid mucopolysaccharides of human brain: identification by means of infra-red analysis. J Neurochem. 1963 Mar;10:165–168. doi: 10.1111/j.1471-4159.1963.tb09478.x. [DOI] [PubMed] [Google Scholar]
  5. Chandrasekaran E. V., Bachhawat B. K. Isolation and characterization of glycosaminoglycans in peripheral nerve and spinal cord of monkey. J Neurochem. 1969 Nov;16(11):1529–1532. doi: 10.1111/j.1471-4159.1969.tb09908.x. [DOI] [PubMed] [Google Scholar]
  6. GROSSFELD H. Positive mucin clot test in supernates of cultures of avian embryonic brain. Proc Soc Exp Biol Med. 1957 Dec;96(3):844–846. doi: 10.3181/00379727-96-23627. [DOI] [PubMed] [Google Scholar]
  7. KIMMEL J. R., SMITH E. L. Crystalline papain. I. Preparation, specificity, and activation. J Biol Chem. 1954 Apr;207(2):515–531. [PubMed] [Google Scholar]
  8. Kraemer P. M. Production of heparin related glycosaminoglycans by an extablished mammalian cell line. J Cell Physiol. 1968 Apr;71(2):109–120. doi: 10.1002/jcp.1040710202. [DOI] [PubMed] [Google Scholar]
  9. LAGUNOFF D., WARREN G. Determination of 2-deoxy-2-sulfoaminohexose content of mucopolysaccharides. Arch Biochem Biophys. 1962 Dec;99:396–400. doi: 10.1016/0003-9861(62)90285-0. [DOI] [PubMed] [Google Scholar]
  10. Margolis R. U. Acid mucopolysaccharides and proteins of bovine whole brain, white matter and myelin. Biochim Biophys Acta. 1967 Jun 13;141(1):91–102. doi: 10.1016/0304-4165(67)90248-6. [DOI] [PubMed] [Google Scholar]
  11. Matalon R., Dorfman A. Hurler's syndrome: biosynthesis of acid mucopolysaccharides in tissue culture. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1310–1316. doi: 10.1073/pnas.56.4.1310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robinson H. C., Dorfman A. The sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses. J Biol Chem. 1969 Jan 25;244(2):348–352. [PubMed] [Google Scholar]
  13. Singh M., Bachhawat B. K. The distribution and variation with age of different uronic acid-containing mucopolysaccharides in brain. J Neurochem. 1965 Jun;12(6):519–525. doi: 10.1111/j.1471-4159.1965.tb06779.x. [DOI] [PubMed] [Google Scholar]
  14. Singh M., Chandrasekaran E. V., Cherian R., Bachhawat B. K. Isolation and characterization of glycosaminoglycans in brain of different species. J Neurochem. 1969 Jul;16(7):1157–1162. doi: 10.1111/j.1471-4159.1969.tb05961.x. [DOI] [PubMed] [Google Scholar]
  15. WOLMAN M. Histochemical study of changes occurring during the degeneration of myelin. J Neurochem. 1957;1(4):370–376. doi: 10.1111/j.1471-4159.1957.tb12095.x. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES