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. 1970 Jul;66(3):595–602. doi: 10.1073/pnas.66.3.595

Dinucleotides as Products of an Exonucleolytic Activity Associated with the Micrococcus luteus DNA Polymerase*

S J Harwood 1, P F Schendel 1,†, L K Miller 1,†, R D Wells 1,‡
PMCID: PMC283091  PMID: 5269226

Abstract

The partially purified DNA polymerase from Micrococcus luteus contains a low level of exonucleolytic activity. The enzyme preparation (1200-fold purified) contains approximately 100 times more polymerase than exonuclease activity. Both single- and double-stranded DNA are degraded at the same rate. The predominant products are dinucleoside diphosphates (d-pXpY); mononucleotides and a trace of trinucleotides are also produced. Each of these products is formed at a constant rate throughout the course of the reaction. The nuclease degrades a DNA chain from the 5′-end. The enzyme preparation contains no detectable endonuclease activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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