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. 1970 Jul;66(3):638–645. doi: 10.1073/pnas.66.3.638

Phosphoglucomutase Mutants of Neurospora sitophila and Their Relation to Morphology*

N C Mishra 1,, E L Tatum 1
PMCID: PMC283098  PMID: 5269231

Abstract

Biochemical and genetic evidence is presented that the rg-1 and rg-2 genes control the structure of the two isozymes of phosphoglucomutase (Phosphoglucomutase I and II) in Neurospora silophila. Results of kinetic and gel filtration studies show that the two phosphoglucomutases may exist in vitro either as separate or as a single but complex molecular species depending on the ionic concentration. The complex phosphoglucomutase molecule is suggested to be the physiologically active form of the enzyme. The change in the enzyme structure is discussed in relation to the morphological change in the ragged mutant strains.

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Selected References

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  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BACHRACH U., COHEN I. Spermidine in the bacterial cell. J Gen Microbiol. 1961 Sep;26:1–9. doi: 10.1099/00221287-26-1-1. [DOI] [PubMed] [Google Scholar]
  3. Brody S., Tatum E. L. Phosphoglucomutase mutants and morphological changes in neurospora crassa. Proc Natl Acad Sci U S A. 1967 Sep;58(3):923–930. doi: 10.1073/pnas.58.3.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHAIKOFF I. L. Metabolic blocks in carbohydrate metabolism in diabetes. Harvey Lect. 1951;Series 47:99–125. [PubMed] [Google Scholar]
  5. Darrow R. A., Rodstrom R. Subunit association and catalytic activity of uridine diphosphate galactose-4-epimerase from yeast. Proc Natl Acad Sci U S A. 1966 Jan;55(1):205–212. doi: 10.1073/pnas.55.1.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dawson D. M., Mitchell A. The isoenzymes of phosphoglucomutase. Biochemistry. 1969 Feb;8(2):609–614. doi: 10.1021/bi00830a022. [DOI] [PubMed] [Google Scholar]
  7. Joshi J. G., Hooper J., Kuwaki T., Sakurada T., Swanson J. R., Handler P. Phosphoglucomutase. V. Multiple forms of phosphoglucomutase. Proc Natl Acad Sci U S A. 1967 May;57(5):1482–1489. doi: 10.1073/pnas.57.5.1482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kirk J. E. The phosphoglucomutase, phosphoglyceric acid mutase, and phosphomannose isomerase activities of arterial tissue in individuals of various ages. J Gerontol. 1966 Jul;21(3):420–425. doi: 10.1093/geronj/21.3.420. [DOI] [PubMed] [Google Scholar]
  9. Mahadevan P. R., Tatum E. L. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol. 1965 Oct;90(4):1073–1081. doi: 10.1128/jb.90.4.1073-1081.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mishra N. C., Threlkeld S. F. Variation in the expression of the ragged mutant in Neurospora. Genetics. 1967 Jan;55(1):113–121. doi: 10.1093/genetics/55.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SPENCER N., HOPKINSON D. A., HARRIS H. PHOSPHOGLUCOMUTASE POLYMORPHISM IN MAN. Nature. 1964 Nov 21;204:742–745. doi: 10.1038/204742a0. [DOI] [PubMed] [Google Scholar]
  12. Schwartz D., Laughner W. J. A molecular basis for heterosis. Science. 1969 Oct 31;166(3905):626–627. doi: 10.1126/science.166.3905.626. [DOI] [PubMed] [Google Scholar]
  13. TSOI A., DOUGLAS H. C. THE EFFECT OF MUTATION OF TWO FORMS OF PHOSPHOGLUCOMUTASE IN SACCHAROMYCES. Biochim Biophys Acta. 1964 Dec 23;92:513–520. doi: 10.1016/0926-6569(64)90011-2. [DOI] [PubMed] [Google Scholar]
  14. Vesterberg O., Svensson H. Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins. Acta Chem Scand. 1966;20(3):820–834. doi: 10.3891/acta.chem.scand.20-0820. [DOI] [PubMed] [Google Scholar]
  15. Wakil S. J., Goldman J. K., Williamson I. P., Toomey R. E. Stimulation of fatty acid biosynthesis by phosphorylated sugars. Proc Natl Acad Sci U S A. 1966 Apr;55(4):880–887. doi: 10.1073/pnas.55.4.880. [DOI] [PMC free article] [PubMed] [Google Scholar]

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