Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jul;66(3):646–650. doi: 10.1073/pnas.66.3.646

Replicative Form II DNA of ϕX174; Resistance to Exonucleolytic Cleavage by E. coli DNA Polymerase

Aharon Razin 1,*, Robert L Sinsheimer 1,†
PMCID: PMC283099  PMID: 4913205

Abstract

The progeny replicative form II DNA isolated from E. coli cells infected with ϕX174 is resistant to the exonucleolytic activity associated with E. coli DNA polymerase. A limited endonucleolytic cleavage with micrococcal endonuclease renders the replicative form II molecule susceptible to the exonucleolytic activity associated with the E. coli DNA polymerase.

These experiments suggest that the resistance of the replicative form II DNA to the exonucleolytic activity is a result of the structure of these molecules at their 5′-terminus.

Full text

PDF
646

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER M., HEPPEL L. A., HURWITZ J. The purification and properties of micrococcal nuclease. J Biol Chem. 1961 Nov;236:3014–3019. [PubMed] [Google Scholar]
  2. Cozzarelli N. R., Kelly R. B., Kornberg A. Enzymic synthesis of DNA. 33. Hydrolysis of a 5'-triphosphate-terminated polynucleotide in the active center of DNA polymerase. J Mol Biol. 1969 Nov 14;45(3):513–531. doi: 10.1016/0022-2836(69)90309-x. [DOI] [PubMed] [Google Scholar]
  3. Deutscher M. P., Kornberg A. Enzymatic synthesis of deoxyribonucleic acid. XXIX. Hydrolysis of deoxyribonucleic acid from the 5' terminus by an exonuclease function of deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):3029–3037. [PubMed] [Google Scholar]
  4. Klett R. P., Cerami A., Reich E. Exonuclease VI, a new nuclease activity associated with E. coli DNA polymerase. Proc Natl Acad Sci U S A. 1968 Jul;60(3):943–950. doi: 10.1073/pnas.60.3.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Knippers R., Razin A., Davis R., Sinsheimer R. L. The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage. J Mol Biol. 1969 Oct 28;45(2):237–263. doi: 10.1016/0022-2836(69)90103-x. [DOI] [PubMed] [Google Scholar]
  6. Kornberg A. Active center of DNA polymerase. Science. 1969 Mar 28;163(3874):1410–1418. doi: 10.1126/science.163.3874.1410. [DOI] [PubMed] [Google Scholar]
  7. ROBERTS W. K., DEKKER C. A., RUSHIZKY G. W., KNIGHT C. A. Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid. Biochim Biophys Acta. 1962 May 14;55:664–673. doi: 10.1016/0006-3002(62)90844-2. [DOI] [PubMed] [Google Scholar]
  8. Wu R., Kaiser A. D. Structure and base sequence in the cohesive ends of bacteriophage lambda DNA. J Mol Biol. 1968 Aug 14;35(3):523–537. doi: 10.1016/s0022-2836(68)80012-9. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES