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. 1970 Jul;66(3):685–692. doi: 10.1073/pnas.66.3.685

Specific Separation of Cells on Affinity Columns*

Paolo Truffa-Bachi 1, Leon Wofsy 1,†
PMCID: PMC283105  PMID: 4913207

Abstract

Cell filtration is performed on affinity columns prepared by the chemical modification of large polyacrylamide beads. Affinity columns selectively bind cells that produce antihapten antibodies of corresponding specificity, while cells without the specific antihapten sites pass through freely and are recovered quantitatively in the eluate. The binding of the antihapten specific cells is inhibited by hapten. Such cell filtration affinity columns may be useful in specifically purifying cell populations with characteristic membrane-receptor proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Corneil I., Wofsy L. Specific purification of equine anti-SII antibodies by precipitation with a hemocyanin-glucuronide conjugate. Immunochemistry. 1967 May;4(3):183–189. doi: 10.1016/0019-2791(67)90127-9. [DOI] [PubMed] [Google Scholar]
  2. Cuatrecasas P., Wilchek M., Anfinsen C. B. Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A. 1968 Oct;61(2):636–643. doi: 10.1073/pnas.61.2.636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Evans W. H., Mage M. G., Peterson E. A. A method for the immunoadsorption of cells to an antibody-coated polyurethane foam. J Immunol. 1969 Apr;102(4):899–907. [PubMed] [Google Scholar]
  4. INGRAHAM J. S. [Specific, complement-dependent hemolysis of sheep erythrocytes by antiserum to azo hapten groups]. J Infect Dis. 1952 Nov-Dec;91(3):268–275. doi: 10.1093/infdis/91.3.268. [DOI] [PubMed] [Google Scholar]
  5. Inman J. K., Dintzis H. M. The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents. Biochemistry. 1969 Oct;8(10):4074–4082. doi: 10.1021/bi00838a026. [DOI] [PubMed] [Google Scholar]
  6. Jerne N. K., Nordin A. A. Plaque Formation in Agar by Single Antibody-Producing Cells. Science. 1963 Apr 26;140(3565):405–405. doi: 10.1126/science.140.3565.405. [DOI] [PubMed] [Google Scholar]
  7. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Wigzell H., Andersson B. Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells. J Exp Med. 1969 Jan 1;129(1):23–36. doi: 10.1084/jem.129.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Wofsy L., Kimura J., Bing D. H., Parker D. C. Affinity labeling of rabbit antisaccharide antibodies. Biochemistry. 1967 Jul;6(7):1981–1988. doi: 10.1021/bi00859a015. [DOI] [PubMed] [Google Scholar]

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