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. 1970 Jul;66(3):730–737. doi: 10.1073/pnas.66.3.730

Proteins Specified by Herpes Simplex Virus, IV. Site of Glycosylation and Accumulation of Viral Membrane Proteins*

Patricia G Spear 1,, Bernard Roizman 1
PMCID: PMC283111  PMID: 4316680

Abstract

The membrane glycoproteins specified by herpes simplex virus are synthesized concurrently with structural viral proteins and accumulate in the cytoplasm and in the membranes lining it. Analyses of free and membranebound polyribosomes, the cytoplasmic pool of soluble proteins, and purified smooth membranes showed that viral membrane proteins bind to membranes soon after synthesis and become glycosylated in situ.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi B., Attardi G. A membrane-associated RNA of cytoplasmic origin in HeLa cells. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1051–1058. doi: 10.1073/pnas.58.3.1051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borun T. W., Scharff M. D., Robbins E. Preparation of mammalian polyribosomes with the detergent Nonidet P-40. Biochim Biophys Acta. 1967 Nov 21;149(1):302–304. doi: 10.1016/0005-2787(67)90715-0. [DOI] [PubMed] [Google Scholar]
  3. Bosmann H. B., Hagopian A., Eylar E. H. Cellular membranes: the isolation and characterization of the plasma and smooth membranes of HeLa cells. Arch Biochem Biophys. 1968 Oct;128(1):51–69. doi: 10.1016/0003-9861(68)90008-8. [DOI] [PubMed] [Google Scholar]
  4. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  5. Keller J. M., Spear P. G., Roizman B. Proteins specified by herpes simplex virus. 3. Viruses differing in their effects on the social behavior of infected cells specify different membrane glycoproteins. Proc Natl Acad Sci U S A. 1970 Apr;65(4):865–871. doi: 10.1073/pnas.65.4.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
  7. Shimono H., Ben-Porat T., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. I. Structural viral proteins. Virology. 1969 Jan;37(1):49–55. doi: 10.1016/0042-6822(69)90305-5. [DOI] [PubMed] [Google Scholar]
  8. Simons K., Käriäinen L. Characterization of the Semliki Forest virus core and envelope protein. Biochem Biophys Res Commun. 1970 Mar 12;38(5):981–988. doi: 10.1016/0006-291x(70)90818-1. [DOI] [PubMed] [Google Scholar]
  9. Spear P. G., Kellejmroian B. Proteins spcified by herpes simplex virus. II. Viral glycoprotins associated with cellular membranes. J Virol. 1970 Feb;5(2):123–131. doi: 10.1128/jvi.5.2.123-131.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spear P. G., Roizman B. An improved procedure for H-3 and C-14 counting in acrylamide gels with a nonaqueous scintillation system. Anal Biochem. 1968 Oct 10;26(1):197–200. doi: 10.1016/0003-2697(68)90048-1. [DOI] [PubMed] [Google Scholar]
  11. Spear P. G., Roizman B. The proteins specified by herpes simplex virus. I. Time of synthesis, transfer into nuclei, and properties of proteins made in productively infected cells. Virology. 1968 Dec;36(4):545–555. doi: 10.1016/0042-6822(68)90186-4. [DOI] [PubMed] [Google Scholar]
  12. Strauss J. H., Jr, Burge B. W., Darnell J. E. Carbohydrate content of the membrane protein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):437–448. doi: 10.1016/0022-2836(70)90313-x. [DOI] [PubMed] [Google Scholar]
  13. WARNER J. R., KNOPF P. M., RICH A. A multiple ribosomal structure in protein synthesis. Proc Natl Acad Sci U S A. 1963 Jan 15;49:122–129. doi: 10.1073/pnas.49.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]

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