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. 1970 Jul;66(3):745–752. doi: 10.1073/pnas.66.3.745

Inactivating and Mutagenic Effects of Nitrosoguanidine on Simian Virus 40*

Peter Tegtmeyer 1,2,, Curt Dohan Jr 1,2, Catherine Reznikoff 1,2
PMCID: PMC283113  PMID: 4316681

Abstract

N-methyl-N′-nitro-N-nitrosoguanidine (NTG) in high concentrations inactivates extracellular simian virus 40. No mutants were found in stocks of virions partially inactivated by NTG under the experimental conditions employed. In contrast, NTG in low concentrations inhibits the intracellular replication of simian virus 40 and is an effective mutagen of the intracellular virus. Six temperature-sensitive mutants of simian virus 40 have been isolated for genetic studies of the virus and its interaction with mammalian cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y., Winocour E., Sachs L. Characterization of the simian virus 40-specific RNA in virus-yielding and transformed cells. J Mol Biol. 1968 Feb 14;31(3):415–429. doi: 10.1016/0022-2836(68)90418-x. [DOI] [PubMed] [Google Scholar]
  2. Anderer F. A., Schlumberger H. D., Koch M. A., Frank H., Eggers H. J. Structure of simian virus 40. II. Symmetry and components of the virus particle. Virology. 1967 Jul;32(3):511–523. doi: 10.1016/0042-6822(67)90303-0. [DOI] [PubMed] [Google Scholar]
  3. Burge B. W., Pfefferkorn E. R. Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology. 1966 Oct;30(2):204–213. doi: 10.1016/0042-6822(66)90096-1. [DOI] [PubMed] [Google Scholar]
  4. CRAWFORD L. V., BLACK P. H. THE NUCLEIC ACID OF SIMIAN VIRUS 40. Virology. 1964 Nov;24:388–392. doi: 10.1016/0042-6822(64)90176-x. [DOI] [PubMed] [Google Scholar]
  5. Cerdá-Olmedo E., Hanawalt P. C. Diazomethane as the active agent in nitrosoguanidine mutagenesis and lethality. Mol Gen Genet. 1968 May 3;101(3):191–202. doi: 10.1007/BF00271621. [DOI] [PubMed] [Google Scholar]
  6. Di Mayorca G., Callender J., Marin G., Giordano R. Temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):126–133. doi: 10.1016/0042-6822(69)90134-2. [DOI] [PubMed] [Google Scholar]
  7. EDDY B. E., BORMAN G. S., GRUBBS G. E., YOUNG R. D. Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40. Virology. 1962 May;17:65–75. doi: 10.1016/0042-6822(62)90082-x. [DOI] [PubMed] [Google Scholar]
  8. Eckhart W. Complementation and transformation by temperature-sensitive mutants of polyoma virus. Virology. 1969 May;38(1):120–125. doi: 10.1016/0042-6822(69)90133-0. [DOI] [PubMed] [Google Scholar]
  9. FRIED M. CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Mar;53:486–491. doi: 10.1073/pnas.53.3.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fields B. N., Joklik W. K. Isolation and preliminary genetic and biochemical characterization of temperature-sensitive mutants of reovirus. Virology. 1969 Mar;37(3):335–342. doi: 10.1016/0042-6822(69)90217-7. [DOI] [PubMed] [Google Scholar]
  11. GIRARDI A. J., SWEET B. H., SLOTNICK V. B., HILLEMAN M. R. Development of tumors in hamsters inoculated in the neonatal period with vacuolating virus, SV-40. Proc Soc Exp Biol Med. 1962 Mar;109:649–660. doi: 10.3181/00379727-109-27298. [DOI] [PubMed] [Google Scholar]
  12. GUENALP A. GROWTH AND CYTOPATHIC EFFECT OF RUBELLA VIRUS IN A LINE OF GREEN MONKEY KIDNEY CELLS. Proc Soc Exp Biol Med. 1965 Jan;118:85–90. [PubMed] [Google Scholar]
  13. Gerber P. Studies on the transfer of subviral infectivity from SV40-induced hamster tumor cells to indicator cells. Virology. 1966 Apr;28(4):501–509. doi: 10.1016/0042-6822(66)90234-0. [DOI] [PubMed] [Google Scholar]
  14. Gharpure M. A., Wright P. F., Chanock R. M. Temperature-sensitive mutants of respiratory syncytial virus. J Virol. 1969 Apr;3(4):414–421. doi: 10.1128/jvi.3.4.414-421.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jerkofsky M., Rapp F. Replication and complementation of human adenoviruses and simian papovavirus at an elevated temperature. J Virol. 1968 Jul;2(7):670–677. doi: 10.1128/jvi.2.7.670-677.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kitahara T., Melnick J. L. Thermal separation of the synthesis of papovavirus SV40 tumor and virus antigens. Proc Soc Exp Biol Med. 1965 Dec;120(3):709–712. doi: 10.3181/00379727-120-30632. [DOI] [PubMed] [Google Scholar]
  17. MANDELL J. D., GREENBERG J. A new chemical mutagen for bacteria, 1-methyl-3-nitro-1-nitrosoguanidine. Biochem Biophys Res Commun. 1960 Dec;3:575–577. doi: 10.1016/0006-291x(60)90064-4. [DOI] [PubMed] [Google Scholar]
  18. Martin M. A., Axelrod D. SV40 gene activity during lytic infection and in a series of SV40 transformed mouse cells. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1203–1210. doi: 10.1073/pnas.64.4.1203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oxman M. N., Baron S., Black P. H., Takemoto K. K., Habel K., Rowe W. P. The effect of interferon on SV-40 T antigen production in SV-40-transformed cells. Virology. 1967 May;32(1):122–127. doi: 10.1016/0042-6822(67)90260-7. [DOI] [PubMed] [Google Scholar]
  21. Pagano J. S., McCutchan J. H., Vaheri A. Factors influencing the enhancement of the infectivity of poliovirus ribonucleic acid by diethylaminoethyl-dextran. J Virol. 1967 Oct;1(5):891–897. doi: 10.1128/jvi.1.5.891-897.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SHEIN H. M., ENDERS J. F., LEVINTHAL J. D. Transformation induced by simian virus 40 in human renal cell cultures. II. Cell-virus relationships. Proc Natl Acad Sci U S A. 1962 Aug;48:1350–1357. doi: 10.1073/pnas.48.8.1350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SHEIN H. M., ENDERS J. F. Transformation induced by simian virus 40 in human renal cell cultures. I. Morphology and growth characteristics. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1164–1172. doi: 10.1073/pnas.48.7.1164. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. SWEET B. H., HILLEMAN M. R. The vacuolating virus, S.V. 40. Proc Soc Exp Biol Med. 1960 Nov;105:420–427. doi: 10.3181/00379727-105-26128. [DOI] [PubMed] [Google Scholar]
  25. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sauer G., Kidwai J. R. The transcription of the SV40 genome in productively infected and transformed cells. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1256–1263. doi: 10.1073/pnas.61.4.1256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Simpson R. W., Hirst G. K. Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology. 1968 May;35(1):41–49. doi: 10.1016/0042-6822(68)90303-6. [DOI] [PubMed] [Google Scholar]
  28. Wallis C., Melnick J. L. Virus aggregation as the cause of the non-neutralizable persistent fraction. J Virol. 1967 Jun;1(3):478–488. doi: 10.1128/jvi.1.3.478-488.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zampieri A., Greenberg J., Warren G. Inactivating and mutagenic effects of 1-methyl-3-nitro-1-nitrosoguanidine on intracellular bacteriophage. J Virol. 1968 Sep;2(9):901–904. doi: 10.1128/jvi.2.9.901-904.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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