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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jul;66(3):787–792. doi: 10.1073/pnas.66.3.787

Temporal Resolution of Individual Steps in an Enzymic Reaction at Low Temperature

P Douzou 1,*, R Sireix 1, F Travers 1
PMCID: PMC283119  PMID: 5269241

Abstract

Media are described which make it possible to study mechanisms of enzyme action at low temperature. The applicability of techniques developed are illustrated by results obtained on formation kinetics of complexes produced by the interaction of horseradish peroxidase with hydrogen peroxide. It is shown that the various steps in the time course of the reaction can be readily resolved with isolation of each intermediate in concentrations sufficient to permit rate studies between consecutive steps. The potential of the method for elaboration of enzyme mechanisms is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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