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. 1970 Jul;66(3):799–806. doi: 10.1073/pnas.66.3.799

Synthesis of Proteins in Cells Infected with Herpesvirus, VI. Characterization of the Proteins of the Viral Membrane*

Albert S Kaplan 1, Tamar Ben-Porat 1
PMCID: PMC283121  PMID: 5269243

Abstract

The envelope of pseudorabies virions can be removed by treatment with Triton X-100, leaving the nucleocapsid intact. The viral envelope removed in this fashion contains all the glycoproteins and about 40 per cent of the proteins of the virions. Analysis by polyacrylamide gel electrophoresis revealed that the envelope proteins migrate as four distinct peaks, all of which contain glycoproteins. The proteins of the nucleocapsids migrate in the gels as six distinct peaks.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  2. Ben-Porat T., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. V. Viral glycoproteins. Virology. 1970 Jun;41(2):265–273. doi: 10.1016/0042-6822(70)90078-4. [DOI] [PubMed] [Google Scholar]
  3. Ben-Porat T., Shimono H., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. II. Flow of structural viral proteins from cytoplasm to nucleus. Virology. 1969 Jan;37(1):56–61. doi: 10.1016/0042-6822(69)90306-7. [DOI] [PubMed] [Google Scholar]
  4. Ben-Porat T., Shimono H., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. IV. Analysis of the proteins in viral particles isolated from the cytoplasm and the nucleus. Virology. 1970 Jun;41(2):256–264. doi: 10.1016/0042-6822(70)90077-2. [DOI] [PubMed] [Google Scholar]
  5. Bosmann H. B., Hagopian A., Eylar E. H. Cellular membranes: the biosynthesis of glycoprotein and glycolipid in hela cell membranes. Arch Biochem Biophys. 1969 Mar;130(1):573–583. doi: 10.1016/0003-9861(69)90073-3. [DOI] [PubMed] [Google Scholar]
  6. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  7. FALKE D., SIEGERT R., VOGELL W. [Electron microscopic findings on the problem of double membrane formation in herpes simplex virus]. Arch Gesamte Virusforsch. 1959;9:484–496. [PubMed] [Google Scholar]
  8. KAPLAN A. S. A study of the herpes simplex virus-rabbit kidney cell system by the plaque technique. Virology. 1957 Dec;4(3):435–457. doi: 10.1016/0042-6822(57)90078-8. [DOI] [PubMed] [Google Scholar]
  9. Kaplan A. S., Shimono H., Ben-Porat T. Synthesis of proteins in cells infected with herpesvirus. 3. Relative amino acid content of various proteins formed after infection. Virology. 1970 Jan;40(1):90–101. doi: 10.1016/0042-6822(70)90382-x. [DOI] [PubMed] [Google Scholar]
  10. Nii S., Morgan C., Rose H. M. Electron microscopy of herpes simplex virus. II. Sequence of development. J Virol. 1968 May;2(5):517–536. doi: 10.1128/jvi.2.5.517-536.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nii S., Morgan C., Rose H. M., Hsu K. C. Electron microscopy of herpes simplex virus. IV. Studies with ferritin-conjugated antibodies. J Virol. 1968 Oct;2(10):1172–1184. doi: 10.1128/jvi.2.10.1172-1184.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olshevsky U., Levitt J., Becker Y. Studies on the synthesis of herpes simplex virions. Virology. 1967 Oct;33(2):323–334. doi: 10.1016/0042-6822(67)90151-1. [DOI] [PubMed] [Google Scholar]
  13. Shimono H., Ben-Porat T., Kaplan A. S. Synthesis of proteins in cells infected with herpesvirus. I. Structural viral proteins. Virology. 1969 Jan;37(1):49–55. doi: 10.1016/0042-6822(69)90305-5. [DOI] [PubMed] [Google Scholar]
  14. Spear P. G., Kellejmroian B. Proteins spcified by herpes simplex virus. II. Viral glycoprotins associated with cellular membranes. J Virol. 1970 Feb;5(2):123–131. doi: 10.1128/jvi.5.2.123-131.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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