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. 1970 Jul;66(3):807–814. doi: 10.1073/pnas.66.3.807

Precipitation of Proteins by Vinblastine and Calcium Ions*

L Wilson 1,†, J Bryan 1,‡, A Ruby 1, D Mazia 1,§
PMCID: PMC283122  PMID: 5269244

Abstract

We have found that vinblastine sulfate can precipitate a number of proteins in addition to microtubule protein. Those proteins which precipitate with vinblastine sulfate, all of which were derived from structure, also precipitate with Ca2+ ions. Our results suggest that vinblastine sulfate, presumably acting as a cation, precipitates proteins by combining with sites which can also combine with Ca2+ ions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bensch K. G., Malawista S. E. Microtubular crystals in mammalian cells. J Cell Biol. 1969 Jan;40(1):95–107. doi: 10.1083/jcb.40.1.95. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borisy G. G., Taylor E. W. The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein. J Cell Biol. 1967 Aug;34(2):525–533. doi: 10.1083/jcb.34.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryan J. The isolation of a major structural element of the sea urchin fertilization membrane. J Cell Biol. 1970 Mar;44(3):635–645. doi: 10.1083/jcb.44.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CUTTS J. H. The effect of vincaleukoblastine on dividing cells in vivo. Cancer Res. 1961 Feb;21:168–172. [PubMed] [Google Scholar]
  5. Creasey W. A., Chou T. C. The binding of colchicine by sarcoma 180 cells. Biochem Pharmacol. 1968 Mar;17(3):477–480. doi: 10.1016/0006-2952(68)90259-1. [DOI] [PubMed] [Google Scholar]
  6. Creasey W. A. Modifications in biochemical pathways produced by the Vinca alkaloids. Cancer Chemother Rep. 1968 Jun;52(4):501–507. [PubMed] [Google Scholar]
  7. Kingsbury E. W., Voelz H. Induction of helical arrays of ribosomes by vinblastine sulfate in Escherichia coli. Science. 1969 Nov 7;166(3906):768–769. doi: 10.1126/science.166.3906.768. [DOI] [PubMed] [Google Scholar]
  8. Krishan A., Hsu D. Observations on the association of helical polyribosomes and filaments with vincristine-induced crystals in Earle's L-cell fibroblasts. J Cell Biol. 1969 Dec;43(3):553–563. doi: 10.1083/jcb.43.3.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. MARTONOSII A., MOLINO C. M., GERGELY J. THE BINDING OF DIVALENT CATIONS TO ACTIN. J Biol Chem. 1964 Apr;239:1057–1064. [PubMed] [Google Scholar]
  11. Malawista S. E., Sato H., Bensch K. G. Vinblastine and griseofulvin reversibly disrupt the living mitotic spindle. Science. 1968 May 17;160(3829):770–772. doi: 10.1126/science.160.3829.770. [DOI] [PubMed] [Google Scholar]
  12. Marantz R., Ventilla M., Shelanski M. Vinblastine-induced precipitation of microtubule protein. Science. 1969 Aug 1;165(3892):498–499. doi: 10.1126/science.165.3892.498. [DOI] [PubMed] [Google Scholar]
  13. Mazia D., Ruby A. Dissolution of erythrocyte membranes in water and comparison of the membrane protein with other structural proteins. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1005–1012. doi: 10.1073/pnas.61.3.1005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Olmsted J. B., Carlson K., Klebe R., Ruddle F., Rosenbaum J. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc Natl Acad Sci U S A. 1970 Jan;65(1):129–136. doi: 10.1073/pnas.65.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. PALMER C. G., LIVENGOOD D., WARREN A. K., SIMPSON P. J., JOHNSON I. S. The action of the vincaleukolastine on mitosis in vitro. Exp Cell Res. 1960 Jun;20:198–201. doi: 10.1016/0014-4827(60)90234-2. [DOI] [PubMed] [Google Scholar]
  16. Palmiter R. D., Christensen A. K., Schimke R. T. Organization of polysomes from pre-existing ribosomes in chick oviduct by a secondary administration of either estradiol or progesterone. J Biol Chem. 1970 Feb 25;245(4):833–845. [PubMed] [Google Scholar]
  17. Richards J. F. Biochemical studies with the Vinca alkaloids. Cancer Chemother Rep. 1968 Jun;52(4):463–467. [PubMed] [Google Scholar]
  18. Sartorelli A. C., Creasey W. A. Cancer chemotherapy. Annu Rev Pharmacol. 1969;9:51–72. doi: 10.1146/annurev.pa.09.040169.000411. [DOI] [PubMed] [Google Scholar]
  19. Wagner E. K., Roizman B. Effect of the Vinca alkaloids on RNA synthesis in human cells in vitro. Science. 1968 Nov 1;162(3853):569–570. doi: 10.1126/science.162.3853.569. [DOI] [PubMed] [Google Scholar]
  20. Weisenberg R. C., Borisy G. G., Taylor E. W. The colchicine-binding protein of mammalian brain and its relation to microtubules. Biochemistry. 1968 Dec;7(12):4466–4479. doi: 10.1021/bi00852a043. [DOI] [PubMed] [Google Scholar]
  21. Wilson L., Friedkin M. The biochemical events of mitosis. I. Synthesis and properties of colchicine labeled with tritium in its acetyl moiety. Biochemistry. 1966 Jul;5(7):2463–2468. doi: 10.1021/bi00871a042. [DOI] [PubMed] [Google Scholar]
  22. Wilson L., Friedkin M. The biochemical events of mitosis. II. The in vivo and in vitro binding of colchicine in grasshopper embryos and its possible relation to inhibition of mitosis. Biochemistry. 1967 Oct;6(10):3126–3135. doi: 10.1021/bi00862a021. [DOI] [PubMed] [Google Scholar]
  23. Wisniewski H., Shelanski M. L., Terry R. D. Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells. J Cell Biol. 1968 Jul;38(1):224–229. doi: 10.1083/jcb.38.1.224. [DOI] [PMC free article] [PubMed] [Google Scholar]

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