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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Jul;66(3):882–889. doi: 10.1073/pnas.66.3.882

Isozyme Patterns and Sexual Morphogenesis in Schizophyllum*

Chiu-Sheng Wang 1,, John R Raper 1,
PMCID: PMC283133  PMID: 4393267

Abstract

Isozymes of several different classes of enzymes in partially purified protein extracts of five strains of Schizophyllum commune, isogenic except for genes controlling sexual morphogenesis, were separated on polyacrylamide gel by disc electrophoresis. After staining, isozyme patterns were compared on the bases of the presence or absence, electrophoretic mobility (Rf values), and relative activities of specific isozymes. Differences in isozyme patterns in 14 enzymes, i.e., NADH-dehydrogenase, NADPH-dehydrogenase, a number of NAD and NADP-dependent dehydrogenases, acid phosphatases, leucine aminopeptidase, and esterases, were correlated with the operation or inactivity of the A-and B-sequences of sexual morphogenesis. In only a single instance, i.e., phenolases, no marked differences could be correlated with sexual morphogenesis.

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Selected References

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