Abstract
The administration of estrogen to immature chicks induces formation of tubular glands and differentiation of cells in the oviduct. As glands begin to form, organized bundles of 40-50 Å filaments appear at the luminal end of the cells. These structures are not present in uninduced oviducts. Cytochalasin treatment of oviducts early in gland formation results in the disappearance of young glands already present and the inhibition of new gland formation. Furthermore, organized microfilaments are no longer present. When the oviducts are washed free of cytochalasin, however, organized bundles of microfilaments reappear.
The correlations between the presence of filaments and formation and glands suggest that filaments are important agents in morphogenesis, presumably because of contractile properties which generate changes in cell shape and, consequently, tissue shape.
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- Baker P. C., Schroeder T. E. Cytoplasmic filaments and morphogenetic movement in the amphibian neural tube. Dev Biol. 1967 May;15(5):432–450. doi: 10.1016/0012-1606(67)90036-x. [DOI] [PubMed] [Google Scholar]
- Carter S. B. Effects of cytochalasins on mammalian cells. Nature. 1967 Jan 21;213(5073):261–264. doi: 10.1038/213261a0. [DOI] [PubMed] [Google Scholar]
- FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kohler P. O., Grimley P. M., O'Malley B. W. Estrogen-induced cytodifferentiation of the ovalbumin-secreting glands of the chick oviduct. J Cell Biol. 1969 Jan;40(1):8–27. doi: 10.1083/jcb.40.1.8. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spooner B. S., Wessells N. K. Effects of cytochalasin B upon microfilaments involved in morphogenesis of salivary epithelium. Proc Natl Acad Sci U S A. 1970 Jun;66(2):360–361. doi: 10.1073/pnas.66.2.360. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szollosi D. Cortical cytoplasmic filaments of cleaving eggs: a structural element corresponding to the contractile ring. J Cell Biol. 1970 Jan;44(1):192–209. doi: 10.1083/jcb.44.1.192. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wrenn J. T., Wessells N. K. An ultrastructural study of lens invagination in the mouse. J Exp Zool. 1969 Jul;171(3):359–367. doi: 10.1002/jez.1401710310. [DOI] [PubMed] [Google Scholar]