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. 1970 Sep;67(1):7–12. doi: 10.1073/pnas.67.1.7

Neurohypophyseal Hormone-Sensitive Adenyl Cyclase of Toad Urinary Bladder*

Hans-Peter Bär 1,2,3,†, Oscar Hechter 1,2,3,‡, Irving L Schwartz 1,2,3, Roderich Walter 1,2,3
PMCID: PMC283157  PMID: 5272332

Abstract

An adenyl cyclase preparation derived from epithelial cells of the urinary bladder of the toad, Bufo marinus, is described. This cyclase preparation is specifically stimulated by neurohypophyseal hormones and various synthetic analogs which evoke a hydroosmotic response in the intact bladder. The relative stimulatory effects of these compounds have been compared on the cyclase preparation and in the intact bladder. The peptide concentrations required for half-maximal stimulation (affinity) in the cell-free and intact systems were parallel; however the magnitude of stimulation produced by saturating concentrations of peptides did not correlate. Furthermore, it was found that peptide analogs which inhibit the hydroosmotic effect of [8-arginine]-vasopressin on the intact bladder also inhibit the stimulation of the toad bladder cyclase preparation by vasopressin. Prostaglandin E1, mercaptans, and disulfides, which inhibit the hormone-induced hydroosmotic response of the intact bladder, did not antagonize the stimulation of the toad bladder cyclase preparation by vasopressin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENTLEY P. J. THE EFFECTS OF N-ETHYLMALEIMIDE AND GLUTATHIONE ON THE ISOLATED RAT UTERUS AND FROG BLADDER WITH SPECIAL REFERENCE TO THE ACTION OF OXYTOCIN. J Endocrinol. 1964 Aug;30:103–113. doi: 10.1677/joe.0.0300103. [DOI] [PubMed] [Google Scholar]
  2. BENTLEY P. J. The effects of neurohypophysial extracts on the water transfer across the wall of the isolated urinary bladder of the toad Bufo marinus. J Endocrinol. 1958 Sep;17(3):201–209. doi: 10.1677/joe.0.0170201. [DOI] [PubMed] [Google Scholar]
  3. BOURGUET J., MAETZ J. [Arguments in favor of the independence of the mechanisms of action of various neurohypophyseal hormones on the osmotic flux of water and on the active transport of sodium in the same receptor; studies on the bladder and the skin of Rana esculenta L]. Biochim Biophys Acta. 1961 Sep 30;52:552–565. doi: 10.1016/0006-3002(61)90414-0. [DOI] [PubMed] [Google Scholar]
  4. Bentley P. J. Natriferic and hydro-osmotic effects on the toad bladder of vasopressin analogues with selective antidiuretic activity. J Endocrinol. 1967 Oct;39(2):299–304. doi: 10.1677/joe.0.0390299. [DOI] [PubMed] [Google Scholar]
  5. Birnbaumer L., Rodbell M. Adenyl cyclase in fat cells. II. Hormone receptors. J Biol Chem. 1969 Jul 10;244(13):3477–3482. [PubMed] [Google Scholar]
  6. Bourgoignie J., Guggenheim S., Kipnis D., Klahr S. Cyclic guanosine monophosphate: effects on short-circuit current and water permeability. Science. 1969 Sep 26;165(3900):1362–1363. doi: 10.1126/science.165.3900.1362. [DOI] [PubMed] [Google Scholar]
  7. Bär H. P., Hechter O. Adenyl cyclase and hormone action. I. Effects of adrenocorticotropic hormone, glucagon, and epinephrine on the plasma membrane of rat fat cells. Proc Natl Acad Sci U S A. 1969 Jun;63(2):350–356. doi: 10.1073/pnas.63.2.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bär H. P., Hechter O. Adenyl cyclase assay in fat cell ghosts. Anal Biochem. 1969 Jun;29(3):476–489. doi: 10.1016/0003-2697(69)90332-7. [DOI] [PubMed] [Google Scholar]
  9. Chase L. R., Aurbach G. D. Renal adenyl cyclase: anatomically separate sites for parathyroid hormone and vasopressin. Science. 1968 Feb 2;159(3814):545–547. doi: 10.1126/science.159.3814.545. [DOI] [PubMed] [Google Scholar]
  10. Dousa T., Hechter O., Walter R., Schwartz I. L. [8-Arginine]-vasopressinoic acid: an inhibitor of rabbit kidney adenyl cyclase. Science. 1970 Feb 20;167(3921):1134–1135. doi: 10.1126/science.167.3921.1134. [DOI] [PubMed] [Google Scholar]
  11. Eggena P., Schwartz I. L., Walter R. A sensitive hydroosmotic toad bladder assay. Affinity and intrinsic activity of neurohypophyseal peptides. J Gen Physiol. 1968 Sep;52(3):465–481. doi: 10.1085/jgp.52.3.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Eggena P., Schwartz I. L., Walter R. Threshold and receptor reserve in the action of neurohypophyseal peptides. A study of synergists and antagonists of the hydroosmotic response of the toad urinary bladder. J Gen Physiol. 1970 Aug;56(2):250–271. doi: 10.1085/jgp.56.2.250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gilman A. G., Rall T. W. Factors influencing adenosine 3',5'-phosphate accumulation in bovine thyroid slices. J Biol Chem. 1968 Nov 25;243(22):5867–5871. [PubMed] [Google Scholar]
  14. Gulyassy P. F., Edelman I. S. Hydrogen-ion dependence of the antidiuretic action of vasopressin, oxytocin and deaminooxytocin. Biochim Biophys Acta. 1965 May 25;102(1):185–197. doi: 10.1016/0926-6585(65)90212-8. [DOI] [PubMed] [Google Scholar]
  15. HANDLER J. S., ORLOFF J. CYSTEINE EFFECT ON TOAD BLADDER RESPONSE TO VASOPRESSIN, CYCLIC AMP, AND THEOPHYLLINE. Am J Physiol. 1964 Mar;206:505–509. doi: 10.1152/ajplegacy.1964.206.3.505. [DOI] [PubMed] [Google Scholar]
  16. Handler J. S., Butcher R. W., Sutherland E. W., Orloff J. The effect of vasopressin and of theophylline on the concentration of adenosine 3',5'-phosphate in the urinary bladder of the toad. J Biol Chem. 1965 Nov;240(11):4524–4526. [PubMed] [Google Scholar]
  17. ORLOFF J., HANDLER J. S., BERGSTROM S. EFFECT OF PROSTAGLANDIN (PGE-1) ON THE PERMEABILITY RESPONSE OF TOAD BLADDER TO VASOPRESSIN, THEOPHYLLINE AND ADENOSINE 3',5'-MONOPHOSPHATE. Nature. 1965 Jan 23;205:397–398. doi: 10.1038/205397a0. [DOI] [PubMed] [Google Scholar]
  18. ORLOFF J., HANDLER J. S. The similarity of effects of vasopressin, adenosine-3',5'-phosphate (cyclic AMP) and theophylline on the toad bladder. J Clin Invest. 1962 Apr;41:702–709. doi: 10.1172/JCI104528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pastan I., Katzen R. Activation of adenyl cyclase in thyroid homogenates by thyroid-stimulating hormone. Biochem Biophys Res Commun. 1967 Dec 29;29(6):792–798. doi: 10.1016/0006-291x(67)90289-6. [DOI] [PubMed] [Google Scholar]
  20. RASMUSSEN H., SCHWARTZ I. L., YOUNG R., MARC-AURELE J. STRUCTURAL REQUIREMENTS FOR THE ACTION OF NEUROHYPOPHYSEAL HORMONES UPON THE ISOLATED AMPHIBIAN URINARY BLADDER. J Gen Physiol. 1963 Jul;46:1171–1189. doi: 10.1085/jgp.46.6.1171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Robison G. A., Butcher R. W., Sutherland E. W. Cyclic AMP. Annu Rev Biochem. 1968;37:149–174. doi: 10.1146/annurev.bi.37.070168.001053. [DOI] [PubMed] [Google Scholar]

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