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. 1970 Sep;67(1):18–25. doi: 10.1073/pnas.67.1.18

Deficient Photosystem II in Agranal Bundle Sheath Chloroplasts of C4 Plants

K C Woo 1,*, Jan M Anderson 1,†, N K Boardman 1,†,‡, W J S Downton 1,*, C B Osmond 1,*, S W Thorne 1,†
PMCID: PMC283159  PMID: 16591853

Abstract

A method is described for separating mesophyll and bundle sheath chloroplasts from the leaves of C4 plants. The agranal bundle sheath chloroplasts are inactive in the Hill reaction, whereas granal bundle sheath and granal mesophyll chloroplasts exhibit normal photosystem II activity. The agranal bundle sheath chloroplasts are deficient in photosystem II; they lack cytochrome b-559 and the fluorescence bands associated with photosystem II. All the chloroplasts exhibit photosystem I activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boardman N. K., Anderson J. M. Fractionation of the photochemical systems of photosynthesis. II. Cytochrome and carotenoid contents of particles isolated from spinach chloroplasts. Biochim Biophys Acta. 1967 Jul 5;143(1):187–203. doi: 10.1016/0005-2728(67)90120-x. [DOI] [PubMed] [Google Scholar]
  2. Boardman N. K., Thorne S. W., Anderson J. M. Fluorescence properties of particles obtained by digitonin fragmentation of spinach chloroplasts. Proc Natl Acad Sci U S A. 1966 Aug;56(2):586–593. doi: 10.1073/pnas.56.2.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boardman N. K., Thorne S. W. Studies on a barley mutant lacking chlorophyll b. II. Fluorescence properties of isolated chloroplasts. Biochim Biophys Acta. 1968 Feb 12;153(2):448–458. doi: 10.1016/0005-2728(68)90086-8. [DOI] [PubMed] [Google Scholar]
  4. Goodenough U. W., Armstrong J. J., Levine R. P. Photosynthetic Properties of ac-31, a Mutant Strain of Chlamydomonas reinhardi Devoid of Chloroplast Membrane Stacking. Plant Physiol. 1969 Jul;44(7):1001–1012. doi: 10.1104/pp.44.7.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Homann P. H., Schmid G. H. Photosynthetic reactions of chloroplasts with unusual structures. Plant Physiol. 1967 Nov;42(11):1619–1632. doi: 10.1104/pp.42.11.1619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Slack C. R., Hatch M. D., Goodchild D. J. Distribution of enzymes in mesophyll and parenchyma-sheath chloroplasts of maize leaves in relation to the C4-dicarboxylic acid pathway of photosynthesis. Biochem J. 1969 Sep;114(3):489–498. doi: 10.1042/bj1140489. [DOI] [PMC free article] [PubMed] [Google Scholar]

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