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. 1970 Sep;67(1):68–73. doi: 10.1073/pnas.67.1.68

Polynucleotide Ligase-Catalyzed Joining of Deoxyribo-oligonucleotides on Ribopolynucleotide Templates and of Ribo-oligonucleotides on Deoxyribopolynucleotide Templates*,†

K Kleppe 1,‡, J H van de Sande 1, H G Khorana 1
PMCID: PMC283168  PMID: 5272330

Abstract

T4 polynucleotide ligase efficiently catalyzes the head-to-tail joining of the ribo-oligoadenylates, r-(pA)8 and r-(pA)10, in the presence of high molecular weight deoxypolythymidylate. The enzyme also catalyzes the joining of deoxy-oligothymidylates, e.g., d-(pT)10, in the presence of ribopolyadenylate. The enzyme failed to bring about the joining of r-(pA)10 when poly r-U was used as the template, although a slow formation of the expected activated intermediate from r-(pA)10 was detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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