Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Sep;67(1):164–171. doi: 10.1073/pnas.67.1.164

Epidermal Growth Factor: High and Low Molecular Weight Forms

John M Taylor 1,2, Stanley Cohen 1,2, William M Mitchell 1,2
PMCID: PMC283183  PMID: 4989828

Abstract

In crude homogenates of the submaxillary gland of the male mouse, epidermal growth factor (EGF) activity was found almost entirely in a high molecular weight complex (about 74,000 daltons). This high molecular weight form may be reversibly dissociated into EGF and an EGF-binding protein. EGF was found to have a molecular weight of approximately 6400. The EGF-binding protein has a molecular weight of 29,000, and shows arginyl-esterase activity.

Full text

PDF
164

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COHEN S., ELLIOTT G. A. The stimulation of epidermal keratinization by a protein isolated from the submaxillary gland of the mouse. J Invest Dermatol. 1963 Jan;40:1–5. doi: 10.1038/jid.1963.1. [DOI] [PubMed] [Google Scholar]
  2. COHEN S. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962 May;237:1555–1562. [PubMed] [Google Scholar]
  3. Chance R. E., Ellis R. M., Bromer W. W. Porcine proinsulin: characterization and amino acid sequence. Science. 1968 Jul 12;161(3837):165–167. doi: 10.1126/science.161.3837.165. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. PURIFICATION OF A NERVE-GROWTH PROMOTING PROTEIN FROM THE MOUSE SALIVARY GLAND AND ITS NEURO-CYTOTOXIC ANTISERUM. Proc Natl Acad Sci U S A. 1960 Mar;46(3):302–311. doi: 10.1073/pnas.46.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen S., Stastny M. Epidermal growth factor. 3. The stimulation of polysome formation in chick embryo epidermis. Biochim Biophys Acta. 1968 Sep 24;166(2):427–437. doi: 10.1016/0005-2787(68)90231-1. [DOI] [PubMed] [Google Scholar]
  6. Cohen S. The stimulation of epidermal proliferation by a specific protein (EGF). Dev Biol. 1965 Dec;12(3):394–407. doi: 10.1016/0012-1606(65)90005-9. [DOI] [PubMed] [Google Scholar]
  7. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  8. Goodwin T. W., Morton R. A. The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochem J. 1946;40(5-6):628–632. doi: 10.1042/bj0400628. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greene L. A., Shooter E. M., Varon S. Subunit interaction and enzymatic activity of mouse 7S nerve growth factor. Biochemistry. 1969 Sep;8(9):3735–3741. doi: 10.1021/bi00837a037. [DOI] [PubMed] [Google Scholar]
  10. Heimberg M., Weinstein I., LeQuire V. S., Cohen S. The induction of fatty liver in neonatal animals by a purified protein (EGF) from mouse submaxillary gland. Life Sci. 1965 Sep;4(17):1625–1633. doi: 10.1016/0024-3205(65)90206-7. [DOI] [PubMed] [Google Scholar]
  11. Hoober J. K., Cohen S. Epidermal growth factor. I. The stimulation of protein and ribonucleic acid synthesis in chick embryo epidermis. Biochim Biophys Acta. 1967 Apr 18;138(2):347–356. [PubMed] [Google Scholar]
  12. Hoober J. K., Cohen S. Epidermal growth factor. II. Increased activity of ribosomes from chick embryo epidermis for cell-free protein synthesis. Biochim Biophys Acta. 1967 Apr 18;138(2):357–368. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Mitchell W. M., Hash J. H. The N,O-diacetylmuramidase of Chalaropsis species. II. Physical properties. J Biol Chem. 1969 Jan 10;244(1):17–21. [PubMed] [Google Scholar]
  15. Schachter M. Kallikreins and kinins. Physiol Rev. 1969 Jul;49(3):509–547. doi: 10.1152/physrev.1969.49.3.509. [DOI] [PubMed] [Google Scholar]
  16. Turkington R. W. The role of epithelial growth factor in mammary gland development in vitro. Exp Cell Res. 1969 Sep;57(1):79–85. doi: 10.1016/0014-4827(69)90369-3. [DOI] [PubMed] [Google Scholar]
  17. YPHANTIS D. A. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964 Mar;3:297–317. doi: 10.1021/bi00891a003. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES