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. 1970 Sep;67(1):263–270. doi: 10.1073/pnas.67.1.263

Genetically Induced Subcellular Mislocation of Neurospora Mitochondrial Malate Dehydrogenase

K D Munkres 1,2, K Benveniste 1,2, J Gorski 1,2, C A Zuiches 1,2
PMCID: PMC283197  PMID: 5272317

Abstract

Among 60 ultraviolet-induced missense mutations of the structural genes that code for mitochondrial malate dehydrogenase (M-MDH, EC 1.1.1.37) of Neurospora crassa, two enzyme phenotypes are observed. In a previously described class (C-mutants), M-MDH is malfunctional because of an abnormal conformation induced by association with mitochondria. We describe here a second class (K-mutants) in which the enzyme is malfunctional because of an altered subcellular location. Thus, although both classes cause lesions in the assimilation of exogenous malate, the nature of the lesions differs. In C-mutants, the enzyme misfunctions because of low affinity for malate but remains mitochondrial-bound as in wild-type. Conversely, K-mutant M-MDH is dispersed throughout the cytoplasm.

Studies of a repressible „glyoxysome” isozyme and a constitutive M-MDH of prototroph and mutants indicate that both isozymes are encoded by the same nuclear structural genes and have polypeptide subunits in common.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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