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. 1970 Sep;67(1):275–281. doi: 10.1073/pnas.67.1.275

The Reovirus Replicative Cycle: Conservation of Parental RNA and Protein*

Samuel C Silverstein †, Michael Schonberg 2, Daniel H Levin 2, George Acs 2
PMCID: PMC283199  PMID: 5272318

Abstract

The fate of parental reovirions in the viral replicative cycle has been analyzed using CsCl density centrifugation. After penetration of L-cells, reovirus is converted from a particle of density 1.39 g/cm3 to a subviral particle of density 1.41 g/cm3. This alteration in density is temporally correlated with the hydrolysis of viral coat proteins and is qualitatively similar when particles are labeled in their RNA or protein. Ten hours after infection, when synthesis of progeny virus is underway, the parental RNA and protein are again found at density 1.39 g/cm3. These data demonstrate conservation of the parental RNA and protein in the subviral particle throughout the replicative cycle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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