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. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305

A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate

Alfred G Gilman 1
PMCID: PMC283204  PMID: 4318781

Abstract

A simple and sensitive assay for adenosine 3′:5′-cyclic monophosphate (cAMP) has been developed that is based on competition for protein binding of the nucleotide, presumably to a cAMP-dependent protein kinase. The nucleotide-protein complex is adsorbed on a cellulose ester filter. Assay conditions are such that a binding constant approaching 10-9 M is obtained, and the assay is thus sensitive to 0.05-0.10 pmol of cAMP.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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