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. 1970 Oct;67(2):598–605. doi: 10.1073/pnas.67.2.598

A Density Label for Membranes

C Fred Fox 1,*, John H Law 1, Norihiro Tsukagoshi 1, Golder Wilson 1
PMCID: PMC283248  PMID: 4943174

Abstract

Bromostearic acid satisfies the essential fatty acid requirement of an unsaturated fatty acid auxotroph of Escherichia coli K12. Over 55% of the fatty acids derived from lipids isolated from membranes of cells grown in bromostearate-supplemented medium contained bromine, as established by gas chromatography and mass spectrometry of the methyl esters. During equilibrium centrifugation in sucrose gradients, the cytoplasmic membranes isolated from bromostearate-grown cells banded at a density approximately 0.06 g/cm3 greater than membranes isolated from oleate- or elaidate-grown cells, affording a complete separation between membranes which contain brominated fatty acids and those which do not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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