Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Oct;67(2):629–636. doi: 10.1073/pnas.67.2.629

DNA Replication in Escherichia coli: Location of Recently Incorporated Thymidine Withi Molecules of High Molecular Weight DNA

V N Iyer 1,*, K G Lark 1
PMCID: PMC283252  PMID: 4943176

Abstract

More than 50% of the [3H]thymidine incorporated during short pulses into the DNA of Escherichia coli 15T- can be extracted by alkali as high molecular weight DNA. Density gradient centrifugation and digestion with exonuclease I suggest that these large pieces of DNA are composed of newly synthesized DNA attached to pre-existing material at the 3′ end of the molecule.

Full text

PDF
629

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APOSHIAN H. V., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem. 1962 Feb;237:519–525. [PubMed] [Google Scholar]
  2. Bird R. E., Lark K. G. Chromosome replication in Escherichia coli 15T- at different growth rates: rate of replication of the chromosome and the rate of formation of small pieces. J Mol Biol. 1970 Apr 28;49(2):343–366. doi: 10.1016/0022-2836(70)90249-4. [DOI] [PubMed] [Google Scholar]
  3. CAIRNS J. The bacterial chromosome and its manner of replication as seen by autoradiography. J Mol Biol. 1963 Mar;6:208–213. doi: 10.1016/s0022-2836(63)80070-4. [DOI] [PubMed] [Google Scholar]
  4. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  5. GUTHRIE G. D., SINSHEIMER R. L. Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963 Jun 25;72:290–297. [PubMed] [Google Scholar]
  6. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  7. HANAWALT P. C., RAY D. S. ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOME. Proc Natl Acad Sci U S A. 1964 Jul;52:125–132. doi: 10.1073/pnas.52.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KORNBERG A. Biologic synthesis of deoxyribonucleic acid. Science. 1960 May 20;131(3412):1503–1508. doi: 10.1126/science.131.3412.1503. [DOI] [PubMed] [Google Scholar]
  9. Kornberg A. Active center of DNA polymerase. Science. 1969 Mar 28;163(3874):1410–1418. doi: 10.1126/science.163.3874.1410. [DOI] [PubMed] [Google Scholar]
  10. LEHMAN I. R., NUSSBAUM A. L. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE). J Biol Chem. 1964 Aug;239:2628–2636. [PubMed] [Google Scholar]
  11. Nuzzo F., Brega A., Falaschi A. DNA replication in mammalian cells. I. The size of newly synthesized helices. Proc Natl Acad Sci U S A. 1970 Apr;65(4):1017–1024. doi: 10.1073/pnas.65.4.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okazaki T., Okazaki R. Mechanism of DNA chain growth. IV. Direction of synthesis of T4 short DNA chains as revealed by exonucleolytic degradation. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1242–1248. doi: 10.1073/pnas.64.4.1242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Richardson C. C. Enzymes in DNA metabolism. Annu Rev Biochem. 1969;38:795–840. doi: 10.1146/annurev.bi.38.070169.004051. [DOI] [PubMed] [Google Scholar]
  14. STRACK H. B., KAISER A. D. ON THE STRUCTURE OF THE ENDS OF LAMBADA DNA. J Mol Biol. 1965 May;12:36–49. doi: 10.1016/s0022-2836(65)80280-7. [DOI] [PubMed] [Google Scholar]
  15. Sugimoto K., Okazaki T., Imae Y., Okazaki R. Mechanism of DNA chain growth. 3. Equal annealing of T4 nascent short DNA chains with the separated complementary strands of the phage DNA. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1343–1350. doi: 10.1073/pnas.63.4.1343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Werner R. Initiation and propagation of growing points in the DNA of phage T4. Cold Spring Harb Symp Quant Biol. 1968;33:501–507. doi: 10.1101/sqb.1968.033.01.058. [DOI] [PubMed] [Google Scholar]
  17. Yudelevich A., Ginsberg B., Hurwitz J. Discontinuous synthesis of DNA during replication. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1129–1136. doi: 10.1073/pnas.61.3.1129. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES