Fig. 3.

Radiographic evaluation of ZA on Ace-1 metastases to long bones. Radiographs of mice were taken 28 days after injection of Ace-1 tumor cells into the left cardiac ventricle. Cortical and trabecular bone lysis (thin arrows) was observed in the metaphyseal regions of long bones of mice that received Ace-1 cancer cells and vehicle (C) compared to mice that received vehicle alone (A). ZA-treated nontumor-bearing mice (B) had a mild increase in radioopacity in the proximal metaphysis of long bones compared to vehicle-treated nontumor-bearing mice (A). Mild trabecular bone loss and intact cortices were present in the delayed treatment group (D), whereas in the prophylactic group (E), intact cortices and increased radio-opacity of bone (thick arrow) was present in the metaphyseal region.