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. 1970 Oct;67(2):951–958. doi: 10.1073/pnas.67.2.951

Mechanism of Polymerization of the Salmonella O-Antigen: Utilization of Lipid-Linked Intermediates

Shiro Kanegasaki 1, Andrew Wright 1
PMCID: PMC283297  PMID: 5289031

Abstract

Cell envelope fractions from Salmonella can utilize exogenous lipid-linked intermediates for the synthesis of polymeric O-antigen. We describe a method for preparing aqueous suspensions of lipid intermediates and show that freezing and thawing of cell envelope-lipid intermediate mixtures is required for efficient synthesis. The lipid intermediates move freely in the hydrophobic environment of the membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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