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. 1970 Oct;67(2):998–1004. doi: 10.1073/pnas.67.2.998

Chloride and Potassium Activities in Luminal Fluid of Turtle Thyroid Follicles as Determined by Selective Ion-Exchanger Microelectrodes

S Y Chow 1,2, D Kunze 1,2, A M Brown 1,2, D M Woodbury 1,2
PMCID: PMC283304  PMID: 5289037

Abstract

Cl- and K+ activities in the follicular lumen and the intraluminal potential of the same lumen were measured simultaneously with specific liquid ion-exchanger and 3 M KCl microelectrodes, respectively, in turtle thyroid glands. The Cl- equilibrium potential between the thyroid interstitium and the lumen calculated from the measured Cl- activities was higher than the directly measured intraluminal potential. These data indicate that Cl- is actively transported out of the follicular lumen in turtle thyroid gland. On the other hand, the calculated K+ equilibrium potential was not different from the directly measured potential, which indicates that K+ is probably distributed passively according to the electrochemical gradients between the interstitial and the luminal compartments in turtle thyroid gland. Results obtained from the specific liquid ion-exchanger microelectrode studies correlate well with those obtained from previous radioautographic observations and chemical determinations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brodsky W. A., Schilb T. P. Ionic mechanisms for sodium and chloride transport across turtle bladders. Am J Physiol. 1966 May;210(5):987–996. doi: 10.1152/ajplegacy.1966.210.5.987. [DOI] [PubMed] [Google Scholar]
  2. Chow S. Y., Jee W. S., Taylor G. N., Woodbury D. M. Radioautographic studies of inulin, sulfate and chloride in rat and guinea pig thyroid glands. Endocrinology. 1965 Nov;77(5):818–824. doi: 10.1210/endo-77-5-818. [DOI] [PubMed] [Google Scholar]
  3. Chow S. Y., Woodbury D. M. Radioactive inulin, sulphate and chloride spaces and electrolytes in the thyroid gland of rats at different ages. J Endocrinol. 1969 Oct;45(2):197–205. doi: 10.1677/joe.0.0450197. [DOI] [PubMed] [Google Scholar]
  4. Chow S. Y., Woodbury D. M. Studies on the stromal, luminal and cellular compartments of the thyroid. Endocrinology. 1965 Nov;77(5):825–840. doi: 10.1210/endo-77-5-825. [DOI] [PubMed] [Google Scholar]
  5. Shagrin J., Young J. A. A micropuncture investigation of the cation content and osmolality of colloid from single rat thyroid acici. Pflugers Arch. 1969;307(3):204–210. doi: 10.1007/BF00592085. [DOI] [PubMed] [Google Scholar]
  6. Walker J. L., Jr, Brown A. M. Unified account of the variable effects of carbon dioxide on nerve cells. Science. 1970 Mar 13;167(3924):1502–1504. doi: 10.1126/science.167.3924.1502. [DOI] [PubMed] [Google Scholar]

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