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. 1970 Oct;67(2):1018–1025. doi: 10.1073/pnas.67.2.1018

Chromosomal Distribution of Rapidly Reannealing DNA in Drosophila melanogaster

Peter M M Rae 1
PMCID: PMC283307  PMID: 5002092

Abstract

Cytological hybridization has been used to localize fractions of rapidly reannealing DNA in salivary chromosomes of Drosophila melanogaster. Complementary RNA of high specific activity was transcribed from hydroxyapatite-fractionated rapidly reannealing sequences and from selected buoyant-density fractions of total DNA. It was then hybridized to chromosome squashes after denaturation of DNA in NaOH. Highly „repeated” DNA sequences were detected over much of the chromosome, but were concentrated in chromocentric heterochromatin. A family of sequences with a low percentage of guanosine plus cytidine was highly concentrated in a particular region within the chromo-center. One „euchromatic” region near the tip of chromosome arm 3L also exhibited a concentration of repeated sequences.

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Selected References

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  1. Birnstiel M. L., Wallace H., Sirlin J. L., Fischberg M. Localization of the ribosomal DNA complements in the nucleolar organizer region of Xenopus laevis. Natl Cancer Inst Monogr. 1966 Dec;23:431–447. [PubMed] [Google Scholar]
  2. Bridges C. B. THE BAR "GENE" A DUPLICATION. Science. 1936 Feb 28;83(2148):210–211. doi: 10.1126/science.83.2148.210. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Burgess R. R. A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem. 1969 Nov 25;244(22):6160–6167. [PubMed] [Google Scholar]
  5. Gall J. G., Macgregor H. C., Kidston M. E. Gene amplification in the oocytes of Dytiscid water beetles. Chromosoma. 1969;26(2):169–187. doi: 10.1007/BF00326453. [DOI] [PubMed] [Google Scholar]
  6. Gall J. G., Pardue M. L. Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U S A. 1969 Jun;63(2):378–383. doi: 10.1073/pnas.63.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. John H. A., Birnstiel M. L., Jones K. W. RNA-DNA hybrids at the cytological level. Nature. 1969 Aug 9;223(5206):582–587. doi: 10.1038/223582a0. [DOI] [PubMed] [Google Scholar]
  8. Jones K. W. Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature. 1970 Mar 7;225(5236):912–915. doi: 10.1038/225912a0. [DOI] [PubMed] [Google Scholar]
  9. Melli M., Bishop J. O. Hybridization between rat liver DNA and complementary RNA. J Mol Biol. 1969 Feb 28;40(1):117–136. doi: 10.1016/0022-2836(69)90300-3. [DOI] [PubMed] [Google Scholar]
  10. Pardu M. L., Gerbi S. A., Eckhardt R. A., Gall J. G. Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma. 1970;29(3):268–290. doi: 10.1007/BF00325943. [DOI] [PubMed] [Google Scholar]
  11. Pardue M. L., Gall J. G. Chromosomal localization of mouse satellite DNA. Science. 1970 Jun 12;168(3937):1356–1358. doi: 10.1126/science.168.3937.1356. [DOI] [PubMed] [Google Scholar]
  12. Pardue M. L., Gall J. G. Molecular hybridization of radioactive DNA to the DNA of cytological preparations. Proc Natl Acad Sci U S A. 1969 Oct;64(2):600–604. doi: 10.1073/pnas.64.2.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Perry R. P., Cheng T. Y., Freed J. J., Greenberg J. R., Kelley D. E., Tartof K. D. Evolution of the transcription unit of ribosomal RNA. Proc Natl Acad Sci U S A. 1970 Mar;65(3):609–616. doi: 10.1073/pnas.65.3.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schweizer E., MacKechnie C., Halvorson H. O. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae. J Mol Biol. 1969 Mar 14;40(2):261–277. doi: 10.1016/0022-2836(69)90474-4. [DOI] [PubMed] [Google Scholar]
  16. Waring M., Britten R. J. Nucleotide sequence repetition: a rapidly reassociating fraction of mouse DNA. Science. 1966 Nov 11;154(3750):791–794. doi: 10.1126/science.154.3750.791. [DOI] [PubMed] [Google Scholar]

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