Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Nov;67(3):1148–1155. doi: 10.1073/pnas.67.3.1148

Breakdown by Streptomycin of Initiation Complexes Formed on Ribosomes of Escherichia coli*

Juan Modolell 1,2, Bernard D Davis 1,2
PMCID: PMC283330  PMID: 4922285

Abstract

Streptomycin induces breakdown of the completed 70S initiation complex on ribosomes of Escherichia coli, but it does not interfere with any step in the formation of the complex. Moreover, it does not appear to interact with the ribosome in any special way during initiation, since the kinetics of breakdown are the same whether streptomycin is added before formation of the initiation complex, or after its completion, or (as previously observed) after formation of a polypeptide. fMet-tRNA is released as such, without chain elongation; it is released from a puromycin-reactive („P”) site. Streptomycin thus appears to distort not only the A site of the ribosome (as suggested earlier) but also the P site.

Full text

PDF
1148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. S., Bretscher M. S., Clark B. F., Marcker K. A. A GTP requirement for binding initiator tRNA to ribosomes. Nature. 1967 Jul 29;215(5100):490–492. doi: 10.1038/215490a0. [DOI] [PubMed] [Google Scholar]
  2. Anderson P., Davies J., Davis B. D. Effect of spectinomycin on polypeptide synthesis in extracts of Escherichia coli. J Mol Biol. 1967 Oct 14;29(1):203–215. doi: 10.1016/0022-2836(67)90191-x. [DOI] [PubMed] [Google Scholar]
  3. Cuzin F., Kretchmer N., Greenberg R. E., Hurwitz R., Chapeville F. Enzymatic hydrolysis of N-substituted aminoacyl-tRNA. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2079–2086. doi: 10.1073/pnas.58.5.2079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Erbe R. W., Nau M. M., Leder P. Translation and translocation of defined RNA messengers. J Mol Biol. 1969 Feb 14;39(3):441–460. doi: 10.1016/0022-2836(69)90137-5. [DOI] [PubMed] [Google Scholar]
  5. Ghosh H. P., Khorana H. G. Studies on polynucleotides, LXXXIV. On the role of ribosomal subunits in protein synthesis. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2455–2461. doi: 10.1073/pnas.58.6.2455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gorini L. The contrasting role of strA and ram gene products in ribosomal functioning. Cold Spring Harb Symp Quant Biol. 1969;34:101–109. doi: 10.1101/sqb.1969.034.01.016. [DOI] [PubMed] [Google Scholar]
  7. Gurgo C., Apirion D., Schlessinger D. Polyribosome metabolism in Escherichia coli treated with chloramphenicol, neomycin, spectinomycin or tetracycline. J Mol Biol. 1969 Oct 28;45(2):205–220. doi: 10.1016/0022-2836(69)90100-4. [DOI] [PubMed] [Google Scholar]
  8. Hershey J. W., Dewey K. F., Thach R. E. Purification and properties of initiation factor f-1. Nature. 1969 Jun 7;222(5197):944–947. doi: 10.1038/222944a0. [DOI] [PubMed] [Google Scholar]
  9. Hershey J. W., Thach R. E. Role of guanosine 5'-triphosphate in the initiation of Peptide synthesis, I. Synthesis of formylmethionyl-puromycin. Proc Natl Acad Sci U S A. 1967 Mar;57(3):759–766. doi: 10.1073/pnas.57.3.759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kaempfer R., Meselson M. Studies of ribosomal subunit exchange. Cold Spring Harb Symp Quant Biol. 1969;34:209–220. doi: 10.1101/sqb.1969.034.01.027. [DOI] [PubMed] [Google Scholar]
  11. Kolakofsky D., Ohta T., Thach R. E. Junction of the 50S ribosomal subunit with the 30S initiation complex. Nature. 1968 Oct 19;220(5164):244–247. doi: 10.1038/220244a0. [DOI] [PubMed] [Google Scholar]
  12. Luzzatto L., Apirion D., Schlessinger D. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis. Proc Natl Acad Sci U S A. 1968 Jul;60(3):873–880. doi: 10.1073/pnas.60.3.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Modolell J., Davis B. D. Mechanism of inhibition of ribosomes by streptomycin. Nature. 1969 Oct 25;224(5217):345–348. doi: 10.1038/224345a0. [DOI] [PubMed] [Google Scholar]
  14. Modolell J., Davis B. D. Rapid inhibition of polypeptide chain extension by streptomycin. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1279–1286. doi: 10.1073/pnas.61.4.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mukundan M. A., Hershey J. W., Dewey K. F., Thach R. E. Binding of formylmethionyl-tRNA to 30S ribosomal sub-units. Nature. 1968 Mar 16;217(5133):1013–1016. doi: 10.1038/2171013a0. [DOI] [PubMed] [Google Scholar]
  16. NIRENBERG M., LEDER P. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES. Science. 1964 Sep 25;145(3639):1399–1407. doi: 10.1126/science.145.3639.1399. [DOI] [PubMed] [Google Scholar]
  17. Ohta T., Sarkar S., Thach R. E. The role of guanosine 5'-triphosphate in the initiation of peptide synthesis. 3. Binding of formylmethionyl-tRNA to ribosomes. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1638–1644. doi: 10.1073/pnas.58.4.1638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sparling P. F., Modolell J., Takeda Y., Davis B. D. Ribosomes from Escherichia coli merodiplods heterozygous for resistance to streptomycin and to spectinomycin. J Mol Biol. 1968 Nov 14;37(3):407–421. doi: 10.1016/0022-2836(68)90111-3. [DOI] [PubMed] [Google Scholar]
  19. Subramanian A. R., Davis B. D., Beller R. J. The ribosome dissociation factor and the ribosome-polysome cycle. Cold Spring Harb Symp Quant Biol. 1969;34:223–230. doi: 10.1101/sqb.1969.034.01.028. [DOI] [PubMed] [Google Scholar]
  20. Vogel Z., Zamir A., Elson D. On the specificity and stability of an enzyme that hydrolyzes N-substituted aminoacyl-transfer RNA's. Proc Natl Acad Sci U S A. 1968 Oct;61(2):701–707. doi: 10.1073/pnas.61.2.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Weisblum B., Davies J. Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev. 1968 Dec;32(4 Pt 2):493–528. [PMC free article] [PubMed] [Google Scholar]
  22. de Groot N., Panet A., Lapidot Y. Enzymatic hydrolysis of peptidyl-tRNA. Biochem Biophys Res Commun. 1968 Apr 5;31(1):37–42. doi: 10.1016/0006-291x(68)90027-2. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES