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. 1970 Nov;67(3):1180–1187. doi: 10.1073/pnas.67.3.1180

Location of the Coat Cistron on the RNA of Phage Qβ

J Hindley 1,2, D H Staples 1,2, M A Billeter 1,2, C Weissmann 1,2
PMCID: PMC283334  PMID: 5274447

Abstract

A new approach for the localization of a cistron on a phage RNA has been developed. The coat protein cistron of phage Qβ was found to begin between the 1100th and 1400th nucleotide from the 5′ terminus of Qβ RNA and therefore lies in the middle of the genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bassel B. A., Jr Cell-free protein synthesis directed by Q beta-RNA and by two specific fragments. Proc Natl Acad Sci U S A. 1968 May;60(1):321–328. doi: 10.1073/pnas.60.1.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Billeter M. A., Dahlberg J. E., Goodman H. M., Hindley J., Weissmann C. Nucleotide sequence analysis of an enzymatically synthesized RNA corresponding to the 5'-terminal region of Q beta RNA. Cold Spring Harb Symp Quant Biol. 1969;34:635–646. doi: 10.1101/sqb.1969.034.01.073. [DOI] [PubMed] [Google Scholar]
  3. Billeter M. A., Dahlberg J. E., Goodman H. M., Hindley J., Weissmann C. Sequence of the first 175 nucleotides from the 5' terminus of Qbeta RNA synthesized in vitro. Nature. 1969 Dec 13;224(5224):1083–1086. doi: 10.1038/2241083a0. [DOI] [PubMed] [Google Scholar]
  4. Feix G., Pollet R., Weissmann C. Replication of viral RNA, XVI. Enzymatic synthesis of infectious virual RNA with noninfectious Q-beta minus strands as template. Proc Natl Acad Sci U S A. 1968 Jan;59(1):145–152. doi: 10.1073/pnas.59.1.145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Garwes D., Sillero A., Ochoa S. Virus-specific proteins in Escherichia coli infected with phage Qb. Biochim Biophys Acta. 1969 Jul 22;186(1):166–172. doi: 10.1016/0005-2787(69)90499-7. [DOI] [PubMed] [Google Scholar]
  6. Goodman H. M., Billeter M. A., Hindley J., Weissmann C. The nucleotide sequence at the 5'-terminus of the Q RNA minus trand. Proc Natl Acad Sci U S A. 1970 Oct;67(2):921–928. doi: 10.1073/pnas.67.2.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hindley J., Staples D. H. Sequence of a ribosome binding site in bacteriophage Q-beta-RNA. Nature. 1969 Dec 6;224(5223):964–967. doi: 10.1038/224964a0. [DOI] [PubMed] [Google Scholar]
  8. Jeppesen P. G., Steitz J. A., Gesteland R. F., Spahr P. F. Gene order in the bacteriophage R17 RNA: 5'-a protein-coat protein-synthetase-3'. Nature. 1970 Apr 18;226(5242):230–237. doi: 10.1038/226230a0. [DOI] [PubMed] [Google Scholar]
  9. Konigsberg W., Maita T., Katze J., Weber K. Amino-acid sequence of the "Qbeta" coat protein. Nature. 1970 Jul 18;227(5255):271–273. doi: 10.1038/227271a0. [DOI] [PubMed] [Google Scholar]
  10. Konings R. N., Ward R., Francke B., Hofschneider P. H. Gene order of RNA bacteriophage M 12. Nature. 1970 May 16;226(5246):604–607. doi: 10.1038/226604a0. [DOI] [PubMed] [Google Scholar]
  11. Lodish H. F. Independent translation of the genes of bacteriophage f2 RNA. J Mol Biol. 1968 Mar 28;32(3):681–685. doi: 10.1016/0022-2836(68)90351-3. [DOI] [PubMed] [Google Scholar]
  12. Pollet R., Knolle P., Weissmann C. Replication of viral RNA. XV. Purification and properties of Q-beta minus strands. Proc Natl Acad Sci U S A. 1967 Aug;58(2):766–773. doi: 10.1073/pnas.58.2.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Spahr P. F., Gesteland R. F. Specific cleavage of bacteriophage R17 RNA by an endonuclease isolated from E. coli MRE-600. Proc Natl Acad Sci U S A. 1968 Mar;59(3):876–883. doi: 10.1073/pnas.59.3.876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Steitz J. A. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969 Dec 6;224(5223):957–964. doi: 10.1038/224957a0. [DOI] [PubMed] [Google Scholar]
  15. Symons R. H. Modified procedure for the synthesis of 32P-labelled ribonucleoside 5'-monophosphates of high specific activity. Biochim Biophys Acta. 1968 Feb 26;155(2):609–610. doi: 10.1016/0005-2787(68)90205-0. [DOI] [PubMed] [Google Scholar]
  16. Vanderheiden B. S., Boszormenyi-Nagy I. Preparation of 32-P-labeled nucleotides. Anal Biochem. 1965 Dec;13(3):496–504. doi: 10.1016/0003-2697(65)90343-x. [DOI] [PubMed] [Google Scholar]

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