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. 1970 Nov;67(3):1207–1213. doi: 10.1073/pnas.67.3.1207

Identification and Origin of Some Chloroplast Aminoacyl-tRNA Synthetases and tRNAs*

Bonnie J Reger 1,†, S A Fairfield 1,‡, J L Epler 1, W Edgar Barnett 1
PMCID: PMC283338  PMID: 5274450

Abstract

Light-grown, wild type Euglena gracilis contains two aminoacyl-tRNA synthetases for both phenylalanine and isoleucine. Only one of the two synthetases for each amino acid is found in isolated chloroplasts, as are the light-induced phenylalanine and isoleucine tRNAs. In each case the light-induced chloroplast tRNAs can only be acylated by the chloroplast synthetases. The chloroplast isoleucyl-tRNA synthetase is light-inducible and cannot be detected in dark-grown cells or in cells of the bleached mutant W3BUL. The presence of the chloroplast phenylalanyl-tRNA synthetase in W3BUL, which contains no chloroplast DNA or structure, indicates that this chloroplast enzyme is synthesized in the cytoplasm and is coded by nuclear genes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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