Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1970 Nov;67(3):1299–1306. doi: 10.1073/pnas.67.3.1299

Altered Nucleic Acids of Antibody-Forming Tissues*

J Russell Little 1,2,†, Helaine A Donahue 1,2,‡
PMCID: PMC283352  PMID: 5274459

Abstract

RNA·DNA hybridization experiments suggest differences between the DNA from the liver and spleen of the same animal (rabbit) and between the RNAs from lymph nodes stimulated by different antigens. A larger proportion of spleen DNA than of liver DNA was complementary to the RNA isolated from antigen-stimulated lymph nodes. No difference between liver and spleen DNA was observed in hybrids formed with RNA from lymph nodes of nonimmune animals. This suggests the presence of unique or redundant genes coding for immunoglobulin polypeptide chains in antibody-forming cells.

Full text

PDF
1299

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attardi G., Huang P. C., Kabat S. Recognition of ribosomal RNA sites in DNA. II. The HeLa cell system. Proc Natl Acad Sci U S A. 1965 Jul;54(1):185–192. doi: 10.1073/pnas.54.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  4. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  5. Brown D. D., Dawid I. B. Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA. Science. 1968 Apr 19;160(3825):272–280. doi: 10.1126/science.160.3825.272. [DOI] [PubMed] [Google Scholar]
  6. Church R. B., McCarthy B. J. Related base sequences in the DNA of simple and complex organisms. II. The interpretation of DNA-RNA hybridization studies with mammalian nucleic acids. Biochem Genet. 1968 Jun;2(1):55–73. doi: 10.1007/BF01458451. [DOI] [PubMed] [Google Scholar]
  7. Church R. B., Storb U., McCarthy B. J., Weiser R. S. Synthesis of specific RNA molecules during immune response in the mouse spleen. J Immunol. 1968 Sep;101(3):399–408. [PubMed] [Google Scholar]
  8. Church R., McCarthy B. J. Changes in nuclear and cytoplasmic RNA in regenerating mouse liver. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1548–1555. doi: 10.1073/pnas.58.4.1548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen E. P. The appearance of new species of RNA in the mouse spleen after immunization as detected by molecular hybridization. Proc Natl Acad Sci U S A. 1967 Mar;57(3):673–680. doi: 10.1073/pnas.57.3.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. DAVIDSON J. D., FEIGELSON P. Practical aspects of internal-sample liquid-scintillation counting. Int J Appl Radiat Isot. 1957 Apr;2(1):1–18. doi: 10.1016/0020-708x(57)90021-2. [DOI] [PubMed] [Google Scholar]
  11. Donahue H. A., Moore V. G., Little J. R. Ribonucleic acid synthesis in rabbit lymph node cells. J Immunol. 1970 Sep;105(3):592–603. [PubMed] [Google Scholar]
  12. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  13. Greenberg L. J., Uhr J. W. DNA-RNA hybridization studies of myeloma tumors in mice. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1878–1882. doi: 10.1073/pnas.58.5.1878. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. HALL B. D., SPIEGELMAN S. Sequence complementarity of T2-DNA and T2-specific RNA. Proc Natl Acad Sci U S A. 1961 Feb 15;47:137–163. doi: 10.1073/pnas.47.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. HELMREICH E., KERN M., EISEN H. N. The secretion of antibody by isolated lymph node cells. J Biol Chem. 1961 Feb;236:464–473. [PubMed] [Google Scholar]
  16. Jacobson E. B., Thorbecke G. J. The proliferative and anamnestic antibody response of rabbit lymphoid cells in vitro. I. Immunological memory in the lymph nodes draining and contralateral to the site of a primary antigen injection. J Exp Med. 1969 Aug 1;130(2):287–297. doi: 10.1084/jem.130.2.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kennel D. Titration of the gene sites on DNA by DNA-RNA hybridization. II. The Escherichia coli chromosome. J Mol Biol. 1968 May 28;34(1):85–103. doi: 10.1016/0022-2836(68)90236-2. [DOI] [PubMed] [Google Scholar]
  18. Kennell D., Kotoulas A. Titration of the gene sites on DNA by DNA-RNA hybridization. I. Problem of measurement. J Mol Biol. 1968 May 28;34(1):71–84. doi: 10.1016/0022-2836(68)90235-0. [DOI] [PubMed] [Google Scholar]
  19. Kimmel C. B. On the RNA in cultured myeloma cells producing immunoglobulin. Biochim Biophys Acta. 1969 Jun 17;182(2):361–374. doi: 10.1016/0005-2787(69)90187-7. [DOI] [PubMed] [Google Scholar]
  20. Kuechler E., Rich A. Two rapidly labeled RNA species in the polysomes of antibody-producing lymphoid tissue. Proc Natl Acad Sci U S A. 1969 Jun;63(2):520–527. doi: 10.1073/pnas.63.2.520. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  22. Mach B., Vassalli P. Biosynthesis of RNA in antibody-producing tissues. Proc Natl Acad Sci U S A. 1965 Sep;54(3):975–982. doi: 10.1073/pnas.54.3.975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moore R. L., McCarthy B. J. Related base sequences in the DNA of simple and complex organisms. 3. Variability in the base sequence of the reduplicated genes for ribosomal RNA in the rabbit. Biochem Genet. 1968 Jun;2(1):75–86. doi: 10.1007/BF01458452. [DOI] [PubMed] [Google Scholar]
  24. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. RITOSSA F. M., SPIEGELMAN S. LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965 Apr;53:737–745. doi: 10.1073/pnas.53.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Raska K., Cohen E. P. RNA in mouse cells exposed to different antigens. Nature. 1968 Feb 24;217(5130):720–723. doi: 10.1038/217720a0. [DOI] [PubMed] [Google Scholar]
  27. Ritossa F. M., Atwood K. C., Spiegelman S. A molecular explanation of the bobbed mutants of Drosophila as partial deficiencies of "ribosomal" DNA. Genetics. 1966 Sep;54(3):819–834. doi: 10.1093/genetics/54.3.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. SLY W. S., ECHOLS H., ADLER J. CONTROL OF VIRAL MESSENGER RNA AFTER LAMBDA PHAGE INFECTION AND INDUCTION. Proc Natl Acad Sci U S A. 1965 Feb;53:378–385. doi: 10.1073/pnas.53.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Savitsky J. P., Stand F. A method for the preparation of mammalian deoxyribonucleic acid. Biochim Biophys Acta. 1966 Feb 21;114(2):419–422. doi: 10.1016/0005-2787(66)90325-x. [DOI] [PubMed] [Google Scholar]
  30. Steiner L. A., Eisen H. N. The relative affinity of antibodies synthesized in the secondary response. J Exp Med. 1967 Dec 1;126(6):1185–1205. doi: 10.1084/jem.126.6.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES