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. 1970 Nov;67(3):1528–1533. doi: 10.1073/pnas.67.3.1528

Introduction of a Heterologous Nucleus into Enucleated Cytoplasms of Cultured Mouse L-Cells

Roger L Ladda 1,2,*,†, Richard D Estensen 1,2,‡
PMCID: PMC283386  PMID: 5274477

Abstract

Mouse L-cells exposed to cytochalasin B undergo random nuclear protrusion and, occasionally, total enucleation. The anucleate cell remains viable for several days. Chick erythrocytes were fused to L-cells with inactivated Sendai virus. The resulting hybrids, containing one or more erythrocyte nuclei in addition to the L-cell nucleus, were treated with cytochalasin B. Through the process of random enucleation, the L-cell nucleus was extruded from some hybrid cells while the erythrocyte nucleus was retained in the hybrid. In other experiments, chick erythrocytes were fused directly to enucleated L-cells. The result in both instances is the introduction of an erythrocyte nucleus into L-cell cytoplasm. The erythrocyte nucleus swells and the hybrid cell is capable of incorporating radioactive uridine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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