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. 1970 Nov;67(3):1580–1587. doi: 10.1073/pnas.67.3.1580

DNA Ligase Mutants of Escherichia coli

Martin Gellert 1, Minnie L Bullock 1
PMCID: PMC283393  PMID: 4922293

Abstract

A procedure is described for the isolation of Escherichia coli mutants with either excess or deficient DNA ligase activity. A mutant that overproduces DNA ligase supports the growth of ligase-defective (gene 30 mutant) T4 phages. Even T4 rII-gene 30 double mutants, which are able to grow in normal E. coli, cannot grow in cells deficient in DNA ligase. A functional DNA ligase, supplied either by the phage or the host, thus seems to be required for T4 growth. An E. coli strain that makes a temperature-sensitive DNA ligase becomes radiation-sensitive at high temperature, but otherwise grows normally and shows no obvious defect in DNA replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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