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. 1970 Dec;67(4):1688–1694. doi: 10.1073/pnas.67.4.1688

Photo-Affinity Labeling of Specific Acetylcholine-Binding Sites on Membranes

Hansruedi Kiefer 1,2, Jon Lindstrom 1,2, Edwin S Lennox 1,2, S J Singer 1,2,*
PMCID: PMC283413  PMID: 5275370

Abstract

Acetylcholinesterase of intact red blood cell membranes and the acetylcholine receptor at the neuromuscular junction of whole-frog sartorius muscle have been irreversibly inactivated by photo-affinity labeling with two quaternary ammonium aryl azides. The inactivation requires that the azides, at the time of their photolytic conversion to highly reactive nitrenes, are reversibly bound to the specific acetylcholine-binding sites.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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