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. Author manuscript; available in PMC: 2011 Apr 25.

Published in final edited form as: Virology. 2010 Feb 18;400(1):93–103. doi: 10.1016/j.virol.2010.01.029

The replication kinetics for WNV^C6/36 and WNV^BHK was nearly identical in primary skin cells. Primary skin cells were inoculated with WNVC6/36 () or WNV^BHK (□) at MOI of (A) 0.01, (B) 1, or (C) 50. At various times post-inoculation, medium was harvested and stored at −70°C. WNV titer in the medium was determined by plaque assays on Vero cell monolayers. The averages ± standard deviations from triplicate wells are shown.

Inline graphic — The replication kinetics for WNV^C6/36 and WNV^BHK was nearly identical in primary skin cells. Primary skin cells were inoculated with WNVC6/36 () or WNV^BHK (□) at MOI of (A) 0.01, (B) 1, or (C) 50. At various times post-inoculation, medium was harvested and stored at −70°C. WNV titer in the medium was determined by plaque assays on Vero cell monolayers. The averages ± standard deviations from triplicate wells are shown.