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Effect of brefeldin A on the expression of FIIHRH in HEK293 cells. Transfection with pcDNA3-FIIHRH and pcDNA3-FIIRHR, metabolic labeling, and analysis was performed as described in Fig. 3. Gels from three experiments were quantitated by densitometry, and the amount of prothrombin present in warfarin-treated cells was 90 ± 1% of that of the vitamin K-treated cells for FHRHR and 30 ± 6% for FIIHRH.
