genetics. For the article ”The presence of p53 mutations in human osteosarcomas correlates with high levels of genomic instability,” by Michael Overholtzer, Pulivarthi H. Rao, Reyna Favis, Xin-Yan Lu, Michael B. Elowitz, Francis Barany, Marc Ladanyi, Richard Gorlick, and Arnold J. Levine, which appeared in issue 20, September 30, 2003, of Proc. Natl. Acad. Sci. USA (100, 11547-11552; first published September 12, 2003; 10.1073/pnas.1934852100), the authors note that several tumor samples in Table 1 were numbered incorrectly. OS17 should have read OS18, OS22 should have read OS24, OS27 should have read OS29, OS28 should have read OS30, and OS31 should have read OS33. This correction does not affect the conclusion that no tumors contain both a mutation of p53 and an amplification of HDM2. The corrected table and its legend appear below.
Table 1. p53 mutation screening.
| Tumor | p53 mutation | SSCP | PCR/LDR | |
|---|---|---|---|---|
| 1 | OS2 | Exon 8, D281H G → C | – | + |
| 2 | OS3 | Exon 8, V272M G → A | – | + |
| 3 | OS6 | Exon 5, R175H G → A | + | + |
| 4 | OS7 | Exon 6, frameshift | + | NT |
| 5 | OS8 | Exon 8, R273H G → A | – | + |
| 6 | OS11 | Exon 5, frameshift, del 17nt | + | NT |
| 7 | OS18 | Exon 6, E224D G → C | + | NT |
| 8 | OS19 | Exon 6, Y220C A → G | + | NT |
| 9 | OS24 | Exon 5, V173M G → A | + | NT |
| 10 | OS29 | Exon 5, deletion codon 190 | + | NT |
| 11 | OS30 | Exon 5, V173G T → G | + | NT |
| 12 | OS33 | Exon 8, R273C C → T | – | + |
SSCP, single-strand conformation polymorphism; LDR, ligase-detection reaction; +, found by technique; –, not found; NT, not tested; det 17nt, deletion of 17 nucleotides.