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. 1992 Oct;66(10):6223–6225. doi: 10.1128/jvi.66.10.6223-6225.1992

Bovine leukemia virus gene expression in vivo.

L Haas 1, T Divers 1, J W Casey 1
PMCID: PMC283677  PMID: 1326668

Abstract

The in vivo transcriptional status of bovine leukemia virus was assessed at three stages of infection during the progression of the disease: aleukemic stage, persistent lymphocytosis, and leukemia/lymphosarcoma. Bovine leukemia virus transcripts could be amplified from total or cytoplasmic enriched lymphocyte RNA by reverse transcription polymerase chain reaction in cells from all but a few aleukemic animals. With primer pairs diagnostic for differentially spliced transcripts (full length-genomic, envelope, tax/rex, and alternatively spliced), a trend toward exclusion of both full-length and envelope RNAs, with retention of the tax/rex message, appears as leukemia/lymphosarcoma develops.

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Selected References

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