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. 1987 Jul;61(7):2304–2306. doi: 10.1128/jvi.61.7.2304-2306.1987

Genetic reassortants for identification of the genome segment coding for the bluetongue virus hemagglutinin.

J A Cowley, B M Gorman
PMCID: PMC283696  PMID: 3035228

Abstract

Two bluetongue virus (BTV) serotypes isolated in Australia and two selected reassortants derived from cells coinfected with these viruses have been used to identify the gene coding for the virus hemagglutinin. The parent viruses had characteristic hemagglutination patterns: BTV type 20 agglutinated sheep erythrocytes only; and BTV type 21 agglutinated sheep, bovine, human, and goose erythrocytes. Analysis of the two virus clones that had reassorted in genes coding for the outer capsid polypeptides demonstrated that hemagglutination and hemagglutination inhibition are functions associated with the outer capsid protein (VP2), which is encoded by genome segment 2.

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Selected References

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  1. BRUBAKER M. M., WEST B., EILLIS R. J. HUMAN BLOOD GROUP INFLUENCE ON REOVIRUS HEMAGGLUTINATION TITERS. Proc Soc Exp Biol Med. 1964 Apr;115:1118–1120. doi: 10.3181/00379727-115-29130. [DOI] [PubMed] [Google Scholar]
  2. CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
  3. Cowley J. A., Barry R. D. Characterization of human parainfluenza viruses. I. The structural proteins of parainfluenza virus 2 and their synthesis in infected cells. J Gen Virol. 1983 Oct;64(Pt 10):2117–2125. doi: 10.1099/0022-1317-64-10-2117. [DOI] [PubMed] [Google Scholar]
  4. Davis A. R., Hiti A. L., Nayak D. P. Influenza defective interfering viral RNA is formed by internal deletion of genomic RNA. Proc Natl Acad Sci U S A. 1980 Jan;77(1):215–219. doi: 10.1073/pnas.77.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EGGERS H. J., GOMATOS P. J., TAMM I. Agglutination of bovine erythrocytes: a general characteristic of reovirus type 3. Proc Soc Exp Biol Med. 1962 Aug-Sep;110:879–881. doi: 10.3181/00379727-110-27679. [DOI] [PubMed] [Google Scholar]
  6. Fields S., Winter G. Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment. Cell. 1982 Feb;28(2):303–313. doi: 10.1016/0092-8674(82)90348-8. [DOI] [PubMed] [Google Scholar]
  7. Franklin R. M. Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504–1511. doi: 10.1073/pnas.55.6.1504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gorman B. M., Taylor J., Walker P. J., Davidson W. L., Brown F. Comparison of bluetongue type 20 with certain viruses of the bluetongue and Eubenangee serological groups of orbiviruses. J Gen Virol. 1981 Dec;57(Pt 2):251–261. doi: 10.1099/0022-1317-57-2-251. [DOI] [PubMed] [Google Scholar]
  9. Gorman B. M., Taylor J., Walker P. J., Young P. R. The isolation of recombinants between related orbiviruses. J Gen Virol. 1978 Nov;41(2):333–342. doi: 10.1099/0022-1317-41-2-333. [DOI] [PubMed] [Google Scholar]
  10. Grubman M. J., Appleton J. A., Letchworth G. J., 3rd Identification of bluetongue virus type 17 genome segments coding for polypeptides associated with virus neutralization and intergroup reactivity. Virology. 1983 Dec;131(2):355–366. doi: 10.1016/0042-6822(83)90503-2. [DOI] [PubMed] [Google Scholar]
  11. Herring A. J., Inglis N. F., Ojeh C. K., Snodgrass D. R., Menzies J. D. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982 Sep;16(3):473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hundley F., Biryahwaho B., Gow M., Desselberger U. Genome rearrangements of bovine rotavirus after serial passage at high multiplicity of infection. Virology. 1985 May;143(1):88–103. doi: 10.1016/0042-6822(85)90099-6. [DOI] [PubMed] [Google Scholar]
  13. Hübschle O. J. Bluetongue virus hemagglutination and its inhibition by specific sera. Arch Virol. 1980;64(2):133–140. doi: 10.1007/BF01318017. [DOI] [PubMed] [Google Scholar]
  14. Kahlon J., Sugiyama K., Roy P. Molecular basis of bluetongue virus neutralization. J Virol. 1983 Dec;48(3):627–632. doi: 10.1128/jvi.48.3.627-632.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Martin S. A., Zweerink H. J. Isolation and characterization of two types of bluetongue virus particles. Virology. 1972 Nov;50(2):495–506. doi: 10.1016/0042-6822(72)90400-x. [DOI] [PubMed] [Google Scholar]
  17. Mertens P. P., Brown F., Sangar D. V. Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology. 1984 May;135(1):207–217. doi: 10.1016/0042-6822(84)90131-4. [DOI] [PubMed] [Google Scholar]
  18. PAVRI K. M. Haemagglutination and haemagglutination-inhibition with African horse-sickness virus. Nature. 1961 Jan 21;189:249–249. doi: 10.1038/189249a0. [DOI] [PubMed] [Google Scholar]
  19. Tokuhisa S., Inaba Y., Miura Y., Sato K., Akashi H., Tsuda T., Shibata I. Hemagglutination of epizootic hemorrhagic disease virus. Arch Virol. 1981;69(3-4):291–294. doi: 10.1007/BF01317344. [DOI] [PubMed] [Google Scholar]
  20. Tokuhisa S., Inaba Y., Miura Y., Sato K. Salt-dependent hemagglutination with bluetongue virus. Arch Virol. 1981;70(1):75–78. doi: 10.1007/BF01320796. [DOI] [PubMed] [Google Scholar]
  21. Verwoerd D. W., Els H. J., De Villiers E. M., Huismans H. Structure of the bluetongue virus capsid. J Virol. 1972 Oct;10(4):783–794. doi: 10.1128/jvi.10.4.783-794.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Verwoerd D. W., Louw H., Oellermann R. A. Characterization of bluetongue virus ribonucleic acid. J Virol. 1970 Jan;5(1):1–7. doi: 10.1128/jvi.5.1.1-7.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Weiner H. L., Ramig R. F., Mustoe T. A., Fields B. N. Identification of the gene coding for the hemagglutinin of reovirus. Virology. 1978 May 15;86(2):581–584. doi: 10.1016/0042-6822(78)90099-5. [DOI] [PubMed] [Google Scholar]
  24. van der Walt N. T. A haemagglutination and haemagglutination inhibition test for bluetongue virus. Onderstepoort J Vet Res. 1980 Jun;47(2):113–117. [PubMed] [Google Scholar]

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