Table 3.

Patient characteristics for 12 AML patients for RT-PCR

Sample	Age/sex	Primary/secondary	De novo/relapsed	Cytogenetics	Flt-3 ITD	% CD34	FAB	Notes
1	53/F	Primary	Relapsed	46, XX, t(5;15;12;12)	Unknown	50	M5
2	56/M	Secondary	De novo	45, XY, t(8;9)(q24;q22),-21	Negative	52	NS	Secondary to chronic eosinophilic leukemia
3	47/M	Primary	De novo	Normal	Negative	68	M5
4	40/F	Primary	De novo	Normal	Normal	90	M5
5	77/F	Primary	De novo	46, XX, der(7)t(1;7)(q25;q31.2)	Negative	87	M4	FLT3 TKD positive
6	59/M	Primary	De novo	Normal	Positive	18	NS
7	64/M	Primary	De novo	Normal	Positive	3	M1
8	39/M	Primary	Relapsed	Normal	Positive	27	M4	NPM positive
9	61/F	Primary	De novo	46, XX, t(10;11)(p11.2–12;q23)	Negative	8	M5	MLL positive
10	39/F	Primary	De novo	Normal	Positive	1	M4	NPM positive
11	57/F	Primary	De novo	Normal	Negative	4	M5	NPM positive
12	46/M	Primary	De novo	Failed to grow	Negative	98	M5	FISH negative for monosomy 5,7 and trisomy 8

Twelve patients were screened by RT-PCR for NuSAP1 and CHAF1b gene expression analysis. The patient characteristics are described.

RT-PCR indicates reverse transcription–polymerase chain reaction; TKD, tyrosine kinase domain; NPM, nucleophosmin; FAB, French-American-British; MLL, mixed lineage leukemia; and FISH, fluorescence in situ hybridization.