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. 1980 Apr;17(4):554–557. doi: 10.1128/aac.17.4.554

Comparison of amoxicillin and ampicillin activities in a continuous culture model of the human urinary bladder.

J D Anderson, K R Johnson, M Y Aird
PMCID: PMC283831  PMID: 6994635

Abstract

Sixteen isolates of Escherichia coli were obtained from women with significant acute urinary tract infections who were subsequently treated with amoxicillin. The activities of amoxicillin and ampicillin against these organisms were compared in urine in a continuous culture apparatus which partly simulated the milieu of the human urinary bladder. After introduction of amoxicillin into the bladder model, mean viable counts for 14 susceptible strains (minimum inhibitory concentration less than or equal to 32 microgram/ml) at 10, 20, 30, and 45 min fell to 34, 8, 0.4, and 0.2% of the original. Corresponding figures for ampicillin were 56, 24, 11, and 2.4%. Viable counts obtained at seven timed intervals up to 2 h were significantly (P = 0.025) lower with amoxicillin than ampicillin. Both antibiotics had a similar activity in conventional disk susceptibility and surface plate minimum inhibitory concentration tests. The realism of the model was confirmed by comparing response to amoxicillin in vivo and in vitro. A serious discrepancy was seen in only one of the 16 cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. D., Eftekhar F., Aird M. Y., Hammond J. Role of bacterial growth rates in the epidemiology and pathogenesis of urinary infections in women. J Clin Microbiol. 1979 Dec;10(6):766–771. doi: 10.1128/jcm.10.6.766-771.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson J. D., Warner M., Forshaw H. L. Studies on the effect of amoxycillin and ampicillin on Enterobacteriaceae in an experimental model of urinary infection. J Antimicrob Chemother. 1975 Jun;1(2):197–204. doi: 10.1093/jac/1.2.197. [DOI] [PubMed] [Google Scholar]
  3. Bodey G. P., Nance J. Amoxicillin: in vitro and pharmacological studies. Antimicrob Agents Chemother. 1972 Apr;1(4):358–362. doi: 10.1128/aac.1.4.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COX C. E., HINMAN F., Jr Experiments with induced bacteriuria, vesical emptying and bacterial growth on the mechanism of bladder defense to infection. J Urol. 1961 Dec;86:739–748. doi: 10.1016/S0022-5347(17)65257-1. [DOI] [PubMed] [Google Scholar]
  5. Eshelman F. N., Spyker D. A. Pharmacokinetics of amoxicillin and ampicillin: crossover study of the effect of food. Antimicrob Agents Chemother. 1978 Oct;14(4):539–543. doi: 10.1128/aac.14.4.539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gilbert D. Comparison of amoxicillin and ampicillin in the treatment of urinary tract infections. J Infect Dis. 1974 Jun;129(0):suppl–suppl:S234. doi: 10.1093/infdis/129.supplement_2.s231. [DOI] [PubMed] [Google Scholar]
  7. Greenwood D., O'Grady F. An in vitro model of the urinary bladder. J Antimicrob Chemother. 1978 Mar;4(2):113–120. doi: 10.1093/jac/4.2.113. [DOI] [PubMed] [Google Scholar]
  8. Hinman F., Jr, Cox C. E. The voiding vesical defense mechanism: the mathematical effect of residual urine, voiding interval and volume on bacteriuria. J Urol. 1966 Oct;96(4):491–498. doi: 10.1016/S0022-5347(17)63297-X. [DOI] [PubMed] [Google Scholar]
  9. O'Grady F., Cattell W. R. Kinetics of urinary tract infection. I. Upper urinary tract. Br J Urol. 1966 Apr;38(2):149–155. doi: 10.1111/j.1464-410x.1966.tb09693.x. [DOI] [PubMed] [Google Scholar]
  10. O'Grady F., Cattell W. R. Kinetics of urinary tract infection. II. The bladder. Br J Urol. 1966 Apr;38(2):156–162. doi: 10.1111/j.1464-410x.1966.tb09694.x. [DOI] [PubMed] [Google Scholar]
  11. Rolinson G. N., Macdonald A. C., Wilson D. A. Bactericidal action of beta-lactam antibiotics on Escherichia coli with particular reference to ampicillin and amoxycillin. J Antimicrob Chemother. 1977 Nov;3(6):541–553. doi: 10.1093/jac/3.6.541. [DOI] [PubMed] [Google Scholar]
  12. Shand D. G., Nimmon C. C., O'Grady F., Cattell W. R. Relation between residual urine volume and response to treatment of urinary infection. Lancet. 1970 Jun 20;760(1):1305–1306. doi: 10.1016/s0140-6736(70)91907-0. [DOI] [PubMed] [Google Scholar]
  13. Sutherland R., Croydon E. A., Rolinson G. N. Amoxycillin: a new semi-synthetic penicillin. Br Med J. 1972 Jul 1;3(5817):13–16. doi: 10.1136/bmj.3.5817.13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sutherland R., Rolinson G. N. -amino-p-hydroxybenzylpenicillin (BRL 2333), a new semisynthetic penicillin: in vitro evaluation. Antimicrob Agents Chemother (Bethesda) 1970;10:411–415. [PubMed] [Google Scholar]

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