Figure 5.
CN− complexes of ferrous and ferric cyt cm552 at pH 7.8: (—) oxidized without CN−, (• • •) ferricytochrome after incubation for 30 min with a 100-fold molar excess of KCN, and (---) dithionite reduction of the CN− complex. All spectra were recorded at pH 7.8 in 20 mM Tris-HCl buffer and 0.02% DDM.