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. 1980 May;17(5):838–841. doi: 10.1128/aac.17.5.838

Rifampin and bacteriocin resistance in Bacteroides fragilis.

K G Mossie, D T Jones, F T Robb, D R Woods
PMCID: PMC283884  PMID: 6156645

Abstract

A low-molecular-weight bacteriocin produced by a Bacteroides fragilis strain inhibited ribonucleic acid polymerase activity in crude extracts of a susceptible B. fragilis indicator strain. A total of 10 rifampin-resistant mutants of the indicator strain were isolated. Nine of the rifampin-resistant mutants were resistant to the bacteriocin, and the other mutant was hypersusceptible. The rifampin- and bacteriocin-resistant mutants all adsorbed approximately the same amount of the bacteriocin as the indicator strain. Two of these rifampin- and bacteriocin-resistant mutants were investigated further, and the polymerase activity in crude extracts of the two mutants was not affected by either rifampin or the bacteriocin. The in vitro ribonucleic acid polymerase activity of the hypersusceptible strain was more susceptible to the bacteriocin than the parent indicator strain was. The bacteriocin-producing strain was susceptible to rifampin but was resistant to its own bacteriocin in vivo. The in vitro ribonucleic acid polymerase activity of the producer strain was only slightly affected by 64 arbitrary units of the bacteriocin. Increasing concentrations of the bacteriocin inhibited ribonucleic acid polymerase extracts of the producer strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman C. M., Sidikaro J., Nomura M. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells. Nat New Biol. 1971 Dec 1;234(48):133–137. doi: 10.1038/newbio234133a0. [DOI] [PubMed] [Google Scholar]
  2. Clark S., Losick R., Pero J. New RNA polymerase from Bacillus subtilis infected with phage PBS2. Nature. 1974 Nov 1;252(5478):21–24. doi: 10.1038/252021a0. [DOI] [PubMed] [Google Scholar]
  3. Eichenlaub R., Winkler U. Purification and mode of action of two bacteriocins produced by Serratia marcesens HY. J Gen Microbiol. 1974 Jul;83(0):83–94. doi: 10.1099/00221287-83-1-83. [DOI] [PubMed] [Google Scholar]
  4. Heil A., Zillig W. Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription. FEBS Lett. 1970 Dec;11(3):165–168. doi: 10.1016/0014-5793(70)80519-1. [DOI] [PubMed] [Google Scholar]
  5. Linn T., Losick R., Sonenshein A. L. Rifampin resistance mutation of Bacillus subtilis altering the electrophoretic mobility of the beta subunit of ribonucleic acid polymerase. J Bacteriol. 1975 Jun;122(3):1387–1390. doi: 10.1128/jb.122.3.1387-1390.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moodie H. L., Woods D. R. Anaerobic R factor transfer in Escherichia coli. J Gen Microbiol. 1973 Jun;76(2):437–440. doi: 10.1099/00221287-76-2-437. [DOI] [PubMed] [Google Scholar]
  7. Moodie H. L., Woods D. R. Isolation of obligate anaerobic faecal bacteria using an anaerobic glove cabinet. S Afr Med J. 1973 Sep 29;47(38):1739–1742. [PubMed] [Google Scholar]
  8. Mossie K. G., Jones D. T., Robb F. T., Woods D. R. Characterization and mode of action of a bacteriocin produced by a Bacteroides fragilis strain. Antimicrob Agents Chemother. 1979 Dec;16(6):724–730. doi: 10.1128/aac.16.6.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nomura M. Colicins and related bacteriocins. Annu Rev Microbiol. 1967;21:257–284. doi: 10.1146/annurev.mi.21.100167.001353. [DOI] [PubMed] [Google Scholar]
  10. Sippel A., Hartmann G. Mode of action of rafamycin on the RNA polymerase reaction. Biochim Biophys Acta. 1968 Mar 18;157(1):218–219. doi: 10.1016/0005-2787(68)90286-4. [DOI] [PubMed] [Google Scholar]
  11. Tocchini-Valentini G. P., Marino P., Colvill A. J. Mutant of E. coli containing an altered DNA-dependent RNA polymerase. Nature. 1968 Oct 19;220(5164):275–276. doi: 10.1038/220275a0. [DOI] [PubMed] [Google Scholar]
  12. Van Tassell R. L., Wilkins T. D. Isolation of auxotrophs of Bacteroides fragilis. Can J Microbiol. 1978 Dec;24(12):1619–1621. doi: 10.1139/m78-260. [DOI] [PubMed] [Google Scholar]
  13. Varel V. H., Bryant M. P. Nutritional features of Bacteroides fragilis subsp. fragilis. Appl Microbiol. 1974 Aug;28(2):251–257. doi: 10.1128/am.28.2.251-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wehrli W., Knüsel F., Schmid K., Staehelin M. Interaction of rifamycin with bacterial RNA polymerase. Proc Natl Acad Sci U S A. 1968 Oct;61(2):667–673. doi: 10.1073/pnas.61.2.667. [DOI] [PMC free article] [PubMed] [Google Scholar]

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