Abstract
Measurements of gentamicin concentrations in serum by the luciferase method are affected by sodium phosphate, glucose, and heparin. The effects of the first two factors can be eliminated by omitting phosphate and increasing the level of glucose in the culture medium. The effects of heparin cannot be easily circumvented, thus precluding use of this agent as an anticoagulant. Bilirubin, which purportedly inhibits measurement of gentamicin in serum by the disk diffusion assay, does not affect the accuracy of the luciferase method.
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Selected References
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- McDonald D. R., Waterworth P. M. Alleged effect of bile constituents on gentamicin assays. Antimicrob Agents Chemother. 1974 Jun;5(6):562–564. doi: 10.1128/aac.5.6.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nilsson L. New rapid bioassay of gentamicin based on luciferase assay of extracellular ATP in bacterial cultures. Antimicrob Agents Chemother. 1978 Dec;14(6):812–816. doi: 10.1128/aac.14.6.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OLSSON P., LAGERGREN H., EK S. The elimination from plasma of intravenous heparin. An experimental study on dogs and humans. Acta Med Scand. 1963 May;173:619–630. doi: 10.1111/j.0954-6820.1963.tb17446.x. [DOI] [PubMed] [Google Scholar]
- Regamey C., Schaberg D., Kirby W. M. Inhibitory effect of heparin on gentamicin concentrations in blood. Antimicrob Agents Chemother. 1972 Apr;1(4):329–332. doi: 10.1128/aac.1.4.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Renshaw S., Cornere B. The effect of bilirubin on the assay of gentamicin. J Clin Pathol. 1974 Jun;27(6):445–446. doi: 10.1136/jcp.27.6.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay for circulating nephrotoxic antibiotics. Antimicrob Agents Chemother (Bethesda) 1970;10:83–90. doi: 10.1128/AAC.10.1.83. [DOI] [PubMed] [Google Scholar]
- Shanson D. C., Daniels J. V. Factors affecting plate assay of gentamicin I diluents. J Antimicrob Chemother. 1975 Jun;1(2):219–227. doi: 10.1093/jac/1.2.219. [DOI] [PubMed] [Google Scholar]