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. 1980 Jul;18(1):50–57. doi: 10.1128/aac.18.1.50

Effect and mechanism of action of aphidicolin on yeast deoxyribonucleic acid polymerases.

P Plevani, G Badaracco, E Ginelli, S Sora
PMCID: PMC283938  PMID: 6774665

Abstract

The antibiotic aphidicolin inhibited in vitro deoxyribonucleic acid synthesis catalyzed by crude yeast extracts and by partially purified yeast deoxyribonucleic acid polymerases. The mechanism of action of aphidicolin on yeast deoxyribonucleic acid polymerase I was noncompetitive with deoxyguanosine 5'-triphosphate, deoxyadenosine 5'-triphosphate, and deoxythymidine 5'-triphosphate and was of the mixed type with deoxycytidine 5'-triphosphate. The relative ratio of enzyme to the template-initiator complex was important for detecting the inhibitory effect of the antibiotic. The inhibition of in vitro deoxyribonucleic acid synthesis by aphidicolin was reversible, and the effect on yeast deoxyribonucleic acid polymerase might have been partially mediated by some supplementary factor(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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