Abstract
Thirty-three isolates of Legionella pneumophila, all except one of which were clinical isolates, were tested against 20 antimicrobial agents by using an agar dilution technique. Erythromycin, rifamp]in, and rosaramycin were the most active agents tested. Aminoglycosides, chloramphenicol, and cefoxitin also inhibited the organisms at low concentrations. Other agents, including moxalactam, cefoperazone, and cephalosporins, exhibited moderate to little activity. Tetracycline, doxycycline and minocyeline were apparently inactivated by charcoal-yeast extract medium. There was slight inoculum dependence noted with most of the antimicrobials tested, particularly the beta-lactam agents. There was no consistent difference in susceptibility between Center for Disease Control-supplied stock strains and recent clinical isolates, but there were marked differences with some agents. Susceptibility testing needs to be standardized in view of the influence of inoculum size, strain variation, and the medium used.
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Selected References
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- Edelstein P. H., Finegold S. M. Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens. J Clin Microbiol. 1979 Aug;10(2):141–143. doi: 10.1128/jcm.10.2.141-143.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelstein P. H., Meyer R. D., Finegold S. M. Isolation of Legionella pneumophila from blood. Lancet. 1979 Apr 7;1(8119):750–751. doi: 10.1016/s0140-6736(79)91207-8. [DOI] [PubMed] [Google Scholar]
- Edelstein P. H., Meyer R. D., Finegold S. M. Isolation of a new serotype of legionnaires' disease bacterium. Lancet. 1978 Dec 2;2(8101):1172–1174. doi: 10.1016/s0140-6736(78)92154-2. [DOI] [PubMed] [Google Scholar]
- Fass R. J., Barnishan J. Minimal inhibitory concentrations of 34 antimicrobial agents for control strains Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853. Antimicrob Agents Chemother. 1979 Nov;16(5):622–624. doi: 10.1128/aac.16.5.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. doi: 10.1128/jcm.10.4.437-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fraser D. W., Wachsmuth I., Bopp C., Feeley J. C., Tsai T. F. Antibiotic treatment of guinea-pigs infected with agent of Legionnaires' disease. Lancet. 1978 Jan 28;1(8057):175–178. doi: 10.1016/s0140-6736(78)90611-6. [DOI] [PubMed] [Google Scholar]
- Fu K. P., Neu H. C. Inactivation of beta-lactam antibiotics by Legionella pneumophila. Antimicrob Agents Chemother. 1979 Nov;16(5):561–564. doi: 10.1128/aac.16.5.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hébert G. A., Moss C. W., McDougal L. K., Bozeman F. M., McKinney R. M., Brenner D. J. The rickettsia-like organisms TATLOCK (1943) and HEBA (1959): bacteria phenotypically similar to but genetically distinct from Legionella pneumophila and the WIGA bacterium. Ann Intern Med. 1980 Jan;92(1):45–52. doi: 10.7326/0003-4819-92-1-45. [DOI] [PubMed] [Google Scholar]
- Kirby B. D., Snyder K. M., Meyer R. D., Finegold S. M. Legionnaires' disease: clinical features of 24 cases. Ann Intern Med. 1978 Sep;89(3):297–309. doi: 10.7326/0003-4819-89-3-297. [DOI] [PubMed] [Google Scholar]
- Ristroph J. D., Hedlund K. W., Allen R. G. Liquid medium for growth of Legionella pneumophila. J Clin Microbiol. 1980 Jan;11(1):19–21. doi: 10.1128/jcm.11.1.19-21.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thornsberry C., Baker C. N., Kirven L. A. In vitro activity of antimicrobial agents on Legionnaires disease bacterium. Antimicrob Agents Chemother. 1978 Jan;13(1):78–80. doi: 10.1128/aac.13.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]