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. 1980 Oct;18(4):493–501. doi: 10.1128/aac.18.4.493

Comparative In Vitro Appraisal of Piperacillin, Including Its Activity Against Salmonella typhi

Roy G Robinson 1, Jean Saunders 1, Robert Cassel 1, Colin S Block 1, Hendrik J Koornhof 1
PMCID: PMC284037  PMID: 6449903

Abstract

Piperacillin was evaluated in vitro against 711 clinical isolates of aerobic and anerobic gram-positive and gram-negative bacteria, including 76 isolates of Salmonella typhi. Piperacillin minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were compared with those of a range of β-lactam, aminoglycoside, and other antimicrobial agents, and inoculum size effects were considered. The relationship between dilution and disk diffusion tests was studied by regression analysis. In addition, piperacillin was assessed in combination with aminoglycoside and other β-lactam drugs. This investigation has confirmed the activity of piperacillin against a broad range of bacteria, including Pseudomonas, Enterobacteriaceae, Neisseria, β-lactamase-negative Haemophilus influenzae, and Staphylococcus aureus as well as enterococci, Bacteroides fragilis, and other anaerobes. All strains of Pseudomonas aeruginosa were inhibited by ≤32 μg/ml or less, demonstrating again the potential usefulness of piperacillin in the treatment of pseudomonal infections. S. typhi proved susceptible to piperacillin, all isolates being inhibited by 1 μg/ml. Inoculum size experiments showed that inocula of 108 CFU resulted in MICs and MBCs appreciably higher than those resulting from inocula of 106 CFU, and inocula of 102 CFU resulted in MICs and MBCs appreciably lower than those resulting from inocula of 104 CFU. Piperacillin was active against all gentamicin-resistant pseudomonads tested, but not against gentamicin-resistant klebsiellas and enterobacters. Combinations of piperacillin with tobramycin and amikacin were consistently synergistic against Pseudomonas and Serratia isolates. Less consistent results were shown when piperacillin was combined with aminoglycosides or cephalothin against Klebsiella and indole-positive Proteus isolates, although synergy was observed in most cases. Occasional antagonistic reactions were encountered with piperacillin-cephalothin or piperacillin-tobramycin combinations against the latter isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Block C. S. Gentamicin-resistant gram-negative bacilli in hospital patients. Part I. Preliminary epidemiological assessment. S Afr Med J. 1978 Mar 18;53(11):391–395. [PubMed] [Google Scholar]
  2. Bodey G. P., Le Blanc B. Piperacillin: in vitro evaluation. Antimicrob Agents Chemother. 1978 Jul;14(1):78–87. doi: 10.1128/aac.14.1.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chanbusarakum P., Murray P. R. Analysis of the interactions between piperacillin, ticarcillin, or carbenicillin and aminoglycoside antibiotics. Antimicrob Agents Chemother. 1978 Sep;14(3):505–506. doi: 10.1128/aac.14.3.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dickinson G. M., Cleary T. J., Hoffman T. A. Comparative evaluation of piperacillin in vitro. Antimicrob Agents Chemother. 1978 Dec;14(6):919–921. doi: 10.1128/aac.14.6.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Evans M. A., Wilson P., Leung T., Williams J. D. Pharmacokinetics of piperacillin following intravenous administration. J Antimicrob Chemother. 1978 May;4(3):255–261. doi: 10.1093/jac/4.3.255. [DOI] [PubMed] [Google Scholar]
  6. Fu K. P., Neu H. C. Piperacillin, a new penicillin active against many bacteria resistant to other penicillins. Antimicrob Agents Chemother. 1978 Mar;13(3):358–367. doi: 10.1128/aac.13.3.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. George W. L., Lewis R. P., Meyer R. D. Susceptibility of cephalothin-resistant gram-negative bacilli to piperacillin, cefuroxime, and other selected antibiotics. Antimicrob Agents Chemother. 1978 Mar;13(3):484–489. doi: 10.1128/aac.13.3.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McGowan J. E., Jr, Terry P. M. Susceptibility of gram-negative aerobic bacilli resistant to carbenicillin in a general hospital to piperacillin and ticarcillin. Antimicrob Agents Chemother. 1979 Jan;15(1):137–139. doi: 10.1128/aac.15.1.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Milne S. E., Waterworth P. M. Piperacillin, a new penicillin with high anti-pseudomonal activity. J Antimicrob Chemother. 1978 May;4(3):247–254. doi: 10.1093/jac/4.3.247. [DOI] [PubMed] [Google Scholar]
  10. Monif G. R., Clark P. R., Shuster J. J., Baer H. Susceptibility of the anaerobic bacteria, group D streptococci, Enterobacteriaceae, and Pseudomonas to semisynthetic penicillins: carbenicillin, piperacillin, and ticarcillin. Antimicrob Agents Chemother. 1978 Nov;14(5):643–649. doi: 10.1128/aac.14.5.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Phaneuf D., Neu H. C. Agar disk diffusion susceptibility characteristics of azlocillin, carbenicillin, mezlocillin, piperacillin, and ticarcillin. Antimicrob Agents Chemother. 1979 Nov;16(5):625–630. doi: 10.1128/aac.16.5.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Scragg J. N. Further experience with amoxycillin in typhoid fever in children. Br Med J. 1976 Oct 30;2(6043):1031–1033. doi: 10.1136/bmj.2.6043.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shah P. P., Briedis D. J., Robson H. G., Conterato J. P. In vitro activity of piperacillin compared with that of carbenicillin, ticarcillin, ampicillin, cephalothin, and cefamandole against Pseudomonas aeruginosa and Enterobacteriaceae. Antimicrob Agents Chemother. 1979 Mar;15(3):346–350. doi: 10.1128/aac.15.3.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thornsberry C., Baker C. N., Jones R. N. In vitro antimicrobial activity of piperacillin and seven other beta-lactam antibiotics against Neisseria gonorrhoeae and Haemophilus influenzae, including beta-lactamase producing strains. J Antimicrob Chemother. 1979 Mar;5(2):137–142. doi: 10.1093/jac/5.2.137. [DOI] [PubMed] [Google Scholar]
  16. Tjandramaga T. B., Mullie A., Verbesselt R., De Schepper P. J., Verbist L. Piperacillin: human pharmacokinetics after intravenous and intramuscular administration. Antimicrob Agents Chemother. 1978 Dec;14(6):829–837. doi: 10.1128/aac.14.6.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ueo K., Fukuoka Y., Hayashi T., Yasuda T., Taki H., Tai M., Watanabe Y., Saikawa I., Mitsuhashi S. In vitro and in vivo antibacterial activity of T-1220, a new semisynthetic penicillin. Antimicrob Agents Chemother. 1977 Oct;12(4):455–460. doi: 10.1128/aac.12.4.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Verbist L. In vitro activity of piperacillin, a new semisynthetic penicillin with an unusually broad spectrum of activity. Antimicrob Agents Chemother. 1978 Mar;13(3):349–357. doi: 10.1128/aac.13.3.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wilkins T. D., Chalgren S. Medium for use in antibiotic susceptibility testing of anaerobic bacteria. Antimicrob Agents Chemother. 1976 Dec;10(6):926–928. doi: 10.1128/aac.10.6.926. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Winston D. J., Wang D., Young L. S., Martin W. J., Hewitt W. L. In vitro studies of piperacilin, a new semisynthetic penicillin. Antimicrob Agents Chemother. 1978 Jun;13(6):944–950. doi: 10.1128/aac.13.6.944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wise R., Andrews J. M., Bedford K. A. Comparison of the in vitro activity of Bay k 4999 and piperacillin, two new antipseudomonal broad-spectrum penicillins, with other beta-lactam drugs. Antimicrob Agents Chemother. 1978 Oct;14(4):549–552. doi: 10.1128/aac.14.4.549. [DOI] [PMC free article] [PubMed] [Google Scholar]

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