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. 1980 Oct;18(4):610–615. doi: 10.1128/aac.18.4.610

Characterization of chloramphenicol-resistant Haemophilus influenzae.

M C Roberts, C D Swenson, L M Owens, A L Smith
PMCID: PMC284058  PMID: 6969577

Abstract

We examined nine chloramphenicol-resistant (minimal inhibitory concentration, greater than or equal to 15 micrograms/ml) Haemophilus influenzae strains isolated in various parts of the world to characterize the genetic and biochemical bases of the resistance; four were type b. All nine contained conjugative plasmids, ranging in molecular weight from 34 x 10(6) to 46 x 10(6), which encoded for resistance to chloramphenicol and tetracycline or chloramphenicol, tetracycline, and ampicillin. Deoxyribonucleic acid homology studies showed that these plasmids were closely related to a previously described ampicillin-resistant plasmid, RSF007, and to each other. All nine isolates and their chloramphenicol-resistant transconjugants produced chloramphenicol acetyltransferase. We conclude that chloramphenicol resistance in these strains of H. influenzae is via plasmid-mediated production of chloramphenicol acetyltransferase.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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