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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Biochemistry. 2010 Feb 23;49(7):1486–1494. doi: 10.1021/bi9016523

Figure 2.

Figure 2

Lineshape and 2H ESEEM data of R1 constructs reconstituted in POPC:POPG vesicles. (A) Schematic of the three-pulse ESEEM experimental design. Three π/2 pulses are separated by the time constants τ and T, with T sequentially delayed by dx increments, resulting in the modulation of the observed spin echo. The time constant τ is adjusted to tune the system for optimal detection of a frequency of interest. (B) Left: X-band first-derivative absorption spectra acquired at room temperature over a scan width of 200 G. Middle: ESEEM data obtained with τ = 224 ns (to optimize detection of 2H) in the presence of D2O. The black trace is the normalized time domain data. The grey segment is the back-calculated dead-time signal (see Methods). Right: frequency domain ESEEM signal. The deuteron (2.24) and phosphorous (5.95) frequencies are indicated in MHz units.