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. 1980 Nov;18(5):687–690. doi: 10.1128/aac.18.5.687

Purification and biochemical properties of beta-lactamase produced by Proteus rettgeri.

M Matsuura, H Nakazawa, M Inoue, S Mitsuhashi
PMCID: PMC284076  PMID: 6969578

Abstract

beta-Lactamase produced by Proteus rettgeri was found to be a typical cephalosporin beta-lactamase on the basis of its substrate hydrolysis profile. The enzyme activity was enhanced by prior treatment with an inducer. The enzyme was purified 166-fold by carboxymethyl-Sephadex column chromatography which indicated that its molecular weight was 42,000 +/- 2,000 and its isoelectric point was 8.7. Cefoperazone, cefoxitin, cefusulodin, cefmetazole, cefotaxime, 6059-S, FK749, YM-09330, carbenicillin, and cloxacillin were stable to this enzyme and possessed the function of competitive inhibition, as shown by their affinity for the beta-lactamase. The enzyme activity was inhibited by iodine, p-chloromerburibenzoate, and HG2+ ion. Clavulanic acid and CP-45899 displayed poor inhibitory activity toward this enzyme. The optimal pH was 8.0, and the optimal temperature was 50 degrees C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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