Abstract
The ability of tetracycline to pass through phospholipid bilayers by diffusion was investigated. Liposomes did not retain enclosed tetracycline. Accumulation of tetracycline was observed with liposomes containing entrapped Tet repressor protein. These results indicate that the drug can pass through lipid bilayers. The antibiotic was also shown to bind to liposomes and isolated phospholipids.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chopra I., Ball P. Transport of antibiotics into bacteria. Adv Microb Physiol. 1982;23:183–240. doi: 10.1016/s0065-2911(08)60338-0. [DOI] [PubMed] [Google Scholar]
- Hillen W., Gatz C., Altschmied L., Schollmeier K., Meier I. Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions. J Mol Biol. 1983 Sep 25;169(3):707–721. doi: 10.1016/s0022-2836(83)80166-1. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McMurry L. M., Cullinane J. C., Petrucci R. E., Jr, Levy S. B. Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli. Antimicrob Agents Chemother. 1981 Sep;20(3):307–313. doi: 10.1128/aac.20.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMurry L., Levy S. B. Two transport systems for tetracycline in sensitive Escherichia coli: critical role for an initial rapid uptake system insensitive to energy inhibitors. Antimicrob Agents Chemother. 1978 Aug;14(2):201–209. doi: 10.1128/aac.14.2.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reynard A. M., Nellis L. F. Uptake of tetracycline by Escherichia coli: lack of binding of tetracycline to the uptake system. Biochem Biophys Res Commun. 1972 Sep 5;48(5):1129–1132. doi: 10.1016/0006-291x(72)90827-3. [DOI] [PubMed] [Google Scholar]
- Szoka F., Jr, Papahadjopoulos D. Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4194–4198. doi: 10.1073/pnas.75.9.4194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tyrrell D. A., Heath T. D., Colley C. M., Ryman B. E. New aspects of liposomes. Biochim Biophys Acta. 1976 Dec 14;457(3-4):259–302. doi: 10.1016/0304-4157(76)90002-2. [DOI] [PubMed] [Google Scholar]